ArticlesAbstractPharmacognosy Research,2010,2,2,107-112.DOI:10.4103/0974-8490.62953Published:May 2010Type:Original ArticleAuthors:Ch. Ramesh, and Manohar G Pattar Author(s) affiliations:Ch. Ramesh, Manohar G Pattar Mycology Laboratory, Department of Botany, Karnatak University, Dharwad 580 003, Karnataka, India Abstract:Methanolic extracts of 6 wild edible mushrooms isolated from the Western Ghats of Karnataka were used in this study. Among the isolates (Lycoperdon perlatum, Cantharellus cibarius, Clavaria vermiculris, Ramaria formosa, Marasmius oreades, Pleurotus pulmonarius), only 4 showed satisfactory results. Quantitative analysis of bioactive components revealed that total phenols are the major bioactive component found in extracts of isolates expressed as mg of GAE per gram of fruit body, which ranged from 3.20 ± 0.05 mg/mL to 6.25 ± 0.08 mg/mL. Average concentration of flavonoid ranged from 0.40 ± 0.052 mg/mL to 2.54 ± 0.08 mg/mL; followed by very small concentration of ascorbic acid (range, 0.06 ± 0.01 mg/mL to 0.16 ± 0.01 mg/mL) in all the isolates. All the isolates showed high phenol and flavonoid content, but ascorbic acid content was found in traces. Antioxidant efficiency by inhibitory concentration on 1,1-Diphenly-2-picrylhydrazyl (DPPH) was found significant when compared to standard antioxidant like Buthylated hydroxyanisol (BHA). The concentration (IC 50 ) ranged from 0.94 ± 0.27 mg/mL to 7.57 ± 0.21 mg/mL. Determination of antimicrobial activity profile of all the isolates tested against a panel of standard pathogenic bacteria and fungi indicated that the concentrations of bioactive components directly influence the antimicrobial capability of the isolates. Agar diffusion assay showed considerable activity against all bacteria. Minimum inhibitory concentration values of the extracts of 4 isolates showed that they are also active even in least concentrations. These results are discussed in relation to therapeutic value of the studied mushrooms. Keywords:Agar diffusion assay, Antioxidant, Bioactive, DPPH mushrooms, in-vitro, Western GhatsView:PDF (1.91 MB) PDF Thumbnails Document Outline Search Document Find Toggle Sidebar Previous Next Page: Fullscreen Print Download Current View Zoom Out Zoom In Automatic Zoom Actual Size Fit Page Full Width 50% 75% 100% 125% 150% 200% More Information Less Information Close Click here to download the PDF file. Images Bioactive components of methanolic extract of mushrooms Lp - Lycoperdon perlatum KeywordsIimmunosuppression Innate IimmunityAgar diffusion assayAntioxidantBioactiveDPPH mushroomsin-vitroWestern Ghats ‹ Bronchodilator activity of Aqueous extract of Stem bark of Ailanthus excelsa Roxb up Antiproliferative Activity and Induction of Apoptosis in Estrogen Receptor-positive and Negative Human Breast Carcinoma Cell lines by Gmelina asiatica Roots ›