Antimicrobial, Anti-inflammatory and Antioxidant Activities of Jatropha multifida L. (Euphorbiaceae)

Kokou Anani; Yao Adjrah; Yaovi Améyapoh; Simplice Damintoti Karou; Amegnona Agbonon; Comlan de Souza; Messanvi Gbeassor

Antimicrobial, Anti-inflammatory and Antioxidant Activities of Jatropha multifida L. (Euphorbiaceae)

Articles

Abstract

Pharmacognosy Research,2016,8,2,142-146.

DOI:10.4103/0974-8490.172657

Published:February 2016

Type:Original Article

Authors:

Author(s) affiliations:

Kokou Anani¹, Yao Adjrah¹, Yaovi Ameyapoh², Simplice Damintoti Karou¹, Amegnona Agbonon³, Comlan de Souza², Messanvi Gbeassor³

¹Laboratoire de Microbiologie et de Contrôle de Qualité des Denrées Alimentaires (LAMICODA); Centre de Recherche et de Formation sur les Plantes Médicinales (CERFOPLAM); Centre de Recherche en Agriculture Biologique et Substances Naturelles (CRABISNA), TOGO.

²Laboratoire de Microbiologie et de Contrôle de Qualité des Denrées Alimentaires (LAMICODA); Centre de Recherche et de Formation sur les Plantes Médicinales (CERFOPLAM), TOGO.

³Centre de Recherche et de Formation sur les Plantes Médicinales (CERFOPLAM); Laboratoire de Physiologie et de Pharmacologie, Faculté des Sciences, Université de Lomé, B.P. 1515, TOGO.

Abstract:

Background: Jatropha multifida is used in Togolease folk medicine for the healing of chronic wounds. Objective: This study aims to investigate antibacterial, anti-inflammatory and antioxidant activities of the leaves ethanolic extract. Materials and Methods: The antimicrobial activity was assayed by National Committee for Clinical Laboratory Standards broth microdilution method on strains of Staphylococcus aureus and Pseudomoas aeruginosa isolated from wounds, whereas the anti-inflammatory activity was performed by carrageenan and histamine induced paw edema method in rat modele. The 2, 2-diphenyl- 1picrylhydrazyl (DPPH) free radical scavenging and ferric reducing antioxidant power (FRAP) were used for the antioxidant activity. Results: The antibacterial assay showed an in vitro growth inhibition of P. aeruginosa and S. aureus in dose-dependent manner, with minimum inhibitory concentration values ranging from 2.5 to 3.12 mg/mL for S. aureus and from 6.25 to 12.5 mg/mL for P. aeruginosa. The maximum paw anti-inflammatory effect occurred after 3 and 5 h administration of histamine and carrageenan, respectively. The DPPH radical scavenging and the FRAP assays yielded weak antioxidant activity. Conclusion: J. multifida possesses antibacterial and anti-inflammatory activities that could justify the use of the plant for the treatment of wounds in the folk medicine. Key words: