Background: Flavonoids, alkaloids, tannins, saponins, and phenolic substances are among the several secondary metabolites that have been identified by phytochemical analysis of Nelumbo nucifera. The hepatoprotective, anti-inflammatory, wound-healing, and antioxidant qualities of these bioactive components are well-known. Since oxidative stress is a major contributor to tissue and cellular damage, these substances antioxidant activity is especially important in reducing it, especially when it comes to organ repairs, wound healing, tissue restoration. Materials and Methods: The purpose of the study was to assess the phytochemical components and potential for tissue restoration of Nelumbo nucifera extract. N. nucifera plants were collected, cleaned, dried in the shade, and ground into powder. Soxhlet extraction was used to extract the powdered material using a hydro-alcoholic solvent. Preliminary phytochemical analysis was undertaken using standard qualitative methods to determine the presence of bioactive components such as alkaloids, flavonoids, phenols, saponins, tannins, glycosides, and terpenoids. The tissue restoration activity was conducted on Wistar albino rats. Results: The qualitative and quantitative analysis of Nelumbo nucifera extract revealed the presence of various bioactive compounds. Total tannin Content of Nelumbo nucifera Extract was found 16.93, 37.34, and 42.44 (μg/mL) for hydro alcoholic, Ethanolic and Aqueous extract. Nelumbo nucifera extract significantly enhanced tissue restoration. Conclusion: Nelumbo nucifera extract is abundant in bioactive phytochemicals, such as flavonoids, alkaloids, tannins, and phenolic compounds, which are recognized for their restorative and antioxidant qualities, as the current study has effectively shown. As demonstrated by the improved healing and regeneration in the treated tissues, the extract demonstrated strong healing and repair activity. According to these results, Nelumbo nucifera may be used therapeutically for wound healing and tissue restoration.
