Chemical and Biological Evaluation of the Aqueous Extract of Peumus boldus Molina (Monimiaceae) Leaves

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Abstract
Pharmacognosy Research,2022,14,1,45-52.
Published:December 2021
Type:Original Article
Authors:
Author(s) affiliations:

Bruna Yuka Koide da Silva1, Aniele da Silva Neves Lopes1, Paulo José Sousa Maia2, Josiana Moreira Mar3, Laiane Souza da Silva4, Edgar Aparecido Sanches5, Geone Maia Corrêa1, Jaqueline de Araújo Bezerra3, Dominique Fernandes de Moura do Carmo1,*

1Instituto de Ciências Exatas e tecnologia, Universidade Federal do Amazonas, Itacoatiara-AM, BRAZIL.

2Instituto Multidisciplinar de Química – Centro Multidisciplinar UFRJ Macaé, Universidade Federal of Rio de Janeiro, Macaé-RJ, BRAZIL.

3Instituto Federal de Educação, Ciência e Tecnologia do Amazonas - (IFAM), Centro, Manaus-AM, BRAZIL.

4Instituto de Química, Universidade Federal do Rio Grande do Sul, Agronomia, Porto Alegre-RS, BRAZIL.

5Departamento de Física, Instituto de Ciências Exatas, Universidade Federal do Amazonas, Coroado, Manaus-AM, BRAZIL.

Abstract:

Background: Traditional medicine has inspired the scientific community to discover natural compounds with the ability to inhibit the enzyme acetylcholinesterase (AChE) and with significant antioxidant capacity. These themes are associated with therapeutic processes to decrease the symptoms of Alzheimer’s disease (AD). Acetylcholine (Ach) is an important neurotransmitter in the central nervous system responsible for transmitting the nervous impulse and increasing intestinal motility and dilation of sphincters in the gastrointestinal tract. As the species Peumus boldus Molina is widely known and used in folk medicine to treat gastrointestinal discomfort, its constituents could have an affinity for AChE and could be used to treat AD. Materials and Methods: This study evaluated the chemical, inhibitory activity of AChE (using the Elmann method and in silico), antioxidant capacity (DPPH, ABTS, FRAP, and oxidation inhibition of the β-carotene/linoleic acid system), and quantification of total leaf phenols from a commercial sample of P. boldus. Results: The mass spectral analysis allowed identification of the alkaloids isoboldine, boldine, clocaurine, laurotetanine, N-methylurotetanine, reticuline, and N-methylcoclaurine. In the in silico prediction test, the alkaloid N-metillaurotetanine was the best acetylcholinesterase enzyme inhibitor, with energy of -10.1 kcal mol-1. Conclusion: These results are of scientific relevance for the discovery of new drugs to treat dementia associated with Alzheimer’s disease and stimulate the continuity of chemical and biological studies (anticholinesterase and antioxidant action) of prepurified fractions and substances isolated from P. boldus leaves.

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Result of molecular docking using the Autodock program for a) reticuline; b) galantamine.

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