Effects of Extraction Solvents on Phytochemical Screening, Cytotoxicity and Anti-Obesity Activities of Selected Sri Lankan Medicinal Plants

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Abstract
Pharmacognosy Research,2021,13,4,246-256.
Published:October 2021
Type:Original Article
Authors:
Author(s) affiliations:

Silva Waruni Randika1,2, Nugara Ruwani Nilushi1,*, Manage Pathmalal2,3, Undugoda Lanka1, Udayanga Dhanushka1, Liyanage Renuka Nilmini4, Galhena Prasanna5

1Department of Biosystems Technology, Faculty of Technology, University of Sri Jayewardenepura, Gangodawila, Nugegoda, SRI LANKA.

2Faculty of Graduate Studies, University of Sri Jayewardenepura, Gangodawila, Nugegoda, SRI LANKA.

3Centre for Water Quality and Algae Research, Department of Zoology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, SRI LANKA.

4Department of Materials and Mechanical Technology, Faculty of Technology, University of Sri Jayewardenepura, Gangodawila, Nugegoda, SRI LANKA.

5Department of Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Kelaniya, SRI LANKA.

Abstract:

Background: Phytoconstituents are known to have an arena of health benefits. The choice of extraction solvents for a desired pharmacological activity is poorly characterized. Objectives: The present study compared the use of different solvents to extract phytoconstituents, evaluate cytotoxicity and anti-obesity activities. Materials and Methods: Leaves, bark, flowers or tubers of fourteen commonly utilized plants in the Sri Lankan Ayurvedic system were used to prepare extracts of distilled water (DW), ethanol or n-hexane. Phytochemical screening, total phenolic and flavonoid contents (TPC and TFC), cytotoxicity and triglyceride assays were performed. Results: DW served as the best solvent to extract the highest yield of phytochemicals followed by ethanol marking hexane as the least efficient solvent. The water extract (WE) of Averrhoa bilimbi leaves and ethanol extract (EE) of Cassia auriculata showed the highest TPC. The TFC was lowest in the hexane extract (HE) of all plants. The WE of plants showed ≥75% cell viability below 500 μg/mL with no potential anti-adipogenic activity. The EE of Justicia adhathoda showed maximum lipid inhibition (88.31%), followed by Aerva lanata (74.65%), and Cyperus rotundus (74.04%) at 100 μg/mL. The EE of Ipomoea aquatica (45.11%), Murrya koenigii (41.04%), and Hemidesmus indicus (32.97%) showed the highest viable anti-adiposity activity at 50 μg/mL. Conclusion: The WE of most plants provided a greater yield of phytoconstituents enabling their safe usage in higher doses during Ayurvedic medication. Ethanol served as a better solvent to extract constituents responsible for anti-adipogenesis and no possible correlation was found between the phenolic content and anti-adiposity.

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The cytotoxic potential of different water extracts (WE) under different concentrations (125, 250, 500 μg/mL). Cell viability was determined by MTS assay. All data are shown as mean ± standard deviation of three independent experiments. Significant difference was identified at *p < 0.05 compared to the control group.

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