Attenuation of Methotrexate‑induced Hepatorenal Damage by Terminalia bellerica Fruit Extract in Experimental Rats

Articles

Abstract
Pharmacognosy Research,2018,10,3,301-308.
Published:July 2018
Type:Original Article
Authors:
Author(s) affiliations:

Prerna Chauhan1, Himanshu Sharma1, Surender Singh1, Yogendra Kumar Gupta1, Uma Kumar2

1Department of Pharmacology, All India Institute of Medical Sciences, New Delhi, INDIA.

2Department of Rheumatology, All India Institute of Medical Sciences, New Delhi, INDIA.

Abstract:

Background: Methotrexate (MTX) is used for numerous malignancies and autoimmune disorders. With such widespread use, MTX‑induced hepatorenal toxicity is an issue of concern that still needs to be addressed. Objective: The aim of the present study is to evaluate the role of Terminalia bellerica extract (TBE) in MTX‑induced hepatorenal toxicity in Wistar albino rats. Materials and Methods: Rats were randomly divided into six groups (n = 6) – received MTX 20 mg/kg intraperitoneally on the 4th day along with pretreatment with different doses of TBE (100 mg/kg, 200 mg/kg, and 400 mg/kg, p.o) given from 1st to 15th day. MTX‑induced hepatorenal toxicity was evaluated by biochemical hepatic and renal parameters along with histopathology and immunohistochemistry. Results: Hepatorenal toxicity induced by MTX was attributed to increased oxidative stress, biochemical liver, and kidney parameters and upregulation of caspase‑3 and nuclear factor kappa B (NFkB). MTX‑treated group observed twofold to threefold rise in aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), and creatinine values–138.49 IU/L, 125.81 IU/L, 63.09 mg/dl, and 1.895 mg/dl, respectively. Groups pretreated with TBE (400 mg/kg) observed a significant decrease (P < 0.001) in oxidative stress and biochemical parameters – AST (63.94 IU/L), ALT (55.98 IU/L), BUN (37.02 mg/dl), and creatinine (1.065 mg/dl). Pretreatment with TBE 400 mg/kg, histopathology of both liver and kidney tissues showed improved architectural damage and immunohistochemistry showed downregulation of increased antigens‑caspase‑3 and NFkB. Conclusion: T. bellerica fruit extract (400 mg/kg) showed significant hepatorenal protection by reducing oxidative stress, elevating serum enzymes, and downregulating the tissue expressions of caspase‑3 and NFkB.

PDF
Current View
Click here to download the PDF file.
Images

High‑performance liquid chromatography chromatogram of Terminalia bellerica

Keywords