Pharmacognosy Research logo
Submit
Pharmacognosy ResearchACADEMIC PUBLISHING
Article

Anti-quorum sensing potential of Adenanthera pavonina

1, 1, 1★★ Corresponding author

  1. 1Yenepoya Research Center, Yenepoya University, University Road, Deralakatte, Mangalore, Karnataka, India

CORRESPONDENCE

Punchappady-Devasya Rekha

Yenepoya Research Center, Yenepoya University, University Road, Deralakatte, Mangalore, Karnataka, India

dydirectoryrc@yenepoya.edu.in

Volume 7, Issue 1 · pp. 105-109 · 2014 · DOI: 10.4103/0974-8490.147220

ABSTRACT

Quorum sensing (QS) in Pseudomonas aeruginosa plays a key role in virulence factor production, biofilm formation and antimicrobial resistance. Because of emerging antimicrobial resistance in P. aeruginosa, there is a need to find an alternate nonantibiotic agent for the control of infections caused by this organism.Objective: To evaluate anti-QS activity of Adenanthera pavonina L., a medicinal plant used in traditional medicine. Preliminary screening for anti-QS activity of ethanol extract of A. pavonina was carried out using Chromobacterium violaceum CV026 biosensor strain and inhibition of QS-regulated violacein production was quantified using C. violaceum ATCC12472. Bioassay guided fractionation of ethanol extract resulted in ethyl acetate fraction (AEF) with strong anti-QS activity and AEF was evaluated for inhibition of QS-regulated pyocyanin production, proteolytic, elastolytic activity, swarming motility and biofilm formation in P. aeruginosaPAO1. AEF, at 0.5 mg/ml, inhibited pyocyanin production completely and at 1 mg/ml of AEF, complete inhibition of proteolytic and elastolytic activities were observed. However, viability of P. aeruginosa PAO1 was not affected at the tested concentrations of AEF as observed by cell count. Swarming motility was inhibited at the concentration of 0.1 mg/ml of AEF. Thin layer chromatography and biosensor overlay of AEF showed violacein inhibition zone at Rf value 0.63. From the results of this study, it can be concluded that A. pavonina extracts can be used as effective anti-QS agents.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Vasavi, H. S., Arun, A. B., & Rekha, P. (2014). Anti-quorum sensing potential of Adenanthera pavonina. Pharmacognosy Research, 7(1), 105-109. https://doi.org/10.4103/0974-8490.147220

    Copyright and licence

    © 2014 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0