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Solicitation of HPLC and HPTLC Techniques for Determination of Rutin from Polyalthia longifolia Thwaites

1★, 2, 2, 3★ Corresponding author

  1. 1Department of Pharmacology and Pharmaceutical Medicinal Chemistry, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra, 2Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan
  2. 2Department of Pharmacology and Pharmaceutical Medicinal Chemistry, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra
  3. 3Department of Pharmacology, Y. B. Chavan College of Pharmacy, Rouzabaugh, Aurangabad, Maharashtra, India

CORRESPONDENCE

Gaurav Mahesh Doshi

Department of Pharmacology and Pharmaceutical Medicinal Chemistry, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra

gaurav.pharmacology@gmail.com

Volume 6, Issue 3 · pp. 234-239 · 2014 · DOI: 10.4103/0974-8490.132601

ABSTRACT

Polyalthia longifolia Thwaites is an important traditional plant in India. Rutin, an active constituent has been reported to possess good amount of pharmacological as well as therapeutic potential. The aim of the present study was to find out by analytical techniques how much percentage of rutin is present in the plant leaves’ ethanolic extract by analytical techniques. Shade dried leaves of Polyalthia longifolia were subjected to cold ethanolic extraction followed by monitoring the isolated rutin high-pressure liquid chromatography (HPLC) and high performance thin layer chromatography (HPTLC) after carrying out preliminary phytochemical screening. Extraction yield was found to be 13.94% w/w. Phytochemical screening of the extract showed the presence of flavonoids, steroids, diterpenoids, alkaloids, saponins, tannins and phenolic compounds and mucilage. From the Rf value, the ethanolic extract was found to be having constituent identical to rutin. By HPTLC and HPLC the amount of rutin was found to be 11.60% w/w and 4.03% w/v, respectively. The active constituent isolated was found to be equal to rutin.

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    Doshi, G. M., Zine, S. P., Chaskar, P. K., & Une, H. D. (2014). Solicitation of HPLC and HPTLC Techniques for Determination of Rutin from Polyalthia longifolia Thwaites. Pharmacognosy Research, 6(3), 234-239. https://doi.org/10.4103/0974-8490.132601

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    © 2014 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0