Spectrophotometric Quantification of Flavonoids in Herbal Material, Crude Extract, and Fractions from Leaves of Eugenia uniflora Linn
1, 1, 1, 1
- 1Department of Pharmaceutical Sciences, Laboratory of Pharmacognosy, UFPE, Recife, Pernambuco, BRAZIL.
CORRESPONDENCE
Luiz Alberto Lira Soares
Department of Pharmaceutical Sciences, Laboratory of Pharmacognosy, UFPE, Recife, Pernambuco, BRAZIL.
Volume 9, Issue 3 · pp. 253-260 · 2017 · DOI: 10.4103/pr.pr_143_16
ABSTRACT
Background: The traditional use of Eugenia uniflora L. (“Pitanga”) is reported due to several properties, which have often been related to its flavonoid content.Objective: The aim was to evaluate analytical procedures for quantification of total flavonoids content (TFCs) by ultraviolet-visible (UV-Vis) spectrophotometry in the herbal material (HM), crude extract (CE), and fractions from leaves of E. uniflora.Materials and Methods: The method for quantification of flavonoids after complexation with aluminum chloride (AlCl3) was evaluated: amount of sample (0.25–1.5 g); solvent (40%–80% ethanol); reaction time and AlCl3concentration (2.5%–7.5%). The procedures by direct dilution (DD) and after acid hydrolysis (AH) were used and validated for HM and CE and applied to the aqueous fraction (AqF), hexane fraction, and ethyl acetate fractions (EAF).Results: The ideal conditions of analysis were ethanol 80% as solvent; 0.5 g of sample; λmax of 408 (DD) and 425 nm (AH); 25 min after addition of AlCl3 5%. The procedures validated for standards and samples showed linearity (R2 > 0.99) with limit of detection and limit of quantification between 0.01 and 0.17 mg/mL (rutin and quercetin); and 0.03 and 0.09 mg/mL (quercetin), for DD and AH, respectively. The procedures were accurate (detect, practice, and repair 90%), and stable under robustness conditions (luminosity, storage, reagents, and equipment). The TFCs in AqF and EAF were 0.65 g% and 17.72 g%, calculated as rutin.Conclusions: UV-Vis methods for quantification of TFC in HM, CE, and fractions from leaves of E. uniflora were suitably validated. Regarding the analysis of fractions, the EAF achieved enrichment of about nine times in the content of flavonoids.
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Ramos, R. T. M., Bezerra, I. C. F., Ferreira, M. R. A., & Soares, L. A. L. (2017). Spectrophotometric Quantification of Flavonoids in Herbal Material, Crude Extract, and Fractions from Leaves of Eugenia uniflora Linn. Pharmacognosy Research, 9(3), 253-260. https://doi.org/10.4103/pr.pr_143_16
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