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Assessments of Antioxidant, Antilipid Peroxidation, and In-vitro Safety of Derris scandens Vine Extracts from Southern Thailand

1, 2, 3, 1

  1. 1Department of Forensic Medicine, Department of Master's Degree Program in Toxicology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, THAILAND.
  2. 2Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, THAILAND.
  3. 3Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, THAILAND.

CORRESPONDENCE

Churdsak Jaikang

Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, THAILAND.

churdsak.j@cmu.ac.th

Volume 11, Issue 1 · pp. 60-66 · 2019 · DOI: 10.4103/pr.pr_141_18

ABSTRACT

Background: Derris scandens (DS) has been used in Thai traditional medicine recipes for musculoskeletal pain relief in Southern Thailand. Changing of terrain and climate affected its phytochemical constituents and led to change antioxidant and toxic properties.Objective: The aim of this study was to study phytochemical contents, antioxidant properties, and toxicity of the DS extracts’ in vitro models.Materials and Methods: The DS ethanolic extract (EE) was partially extracted with chloroform (CE), ethyl acetate (EAE), and water (aqueous extract [AE]). Phytochemical contents, antioxidant properties, and toxicity in Caco‑2 cells, peripheral blood mononuclear cells (PBMCs), and red blood cells (RBCs) were explored.Results: Genistein and gallic acid were rich in the EE and CE. The CE demonstrated the highest 2,2 − diphenyl − 1 − picrylhydrazyl scavenging activity with half‑maximal Radical cavenging concentration (SC50) value – 0.81 ± 0.07 mg/mL. The EE showed the highest 2, 2’−azino − bis (3 − ethylbenzothiazoline − 6 − sulfonic acid) radical scavenging activity (SC50 = 22.05 ± 3.91 μg/mL) and all the extracts strongly inhibited lipid peroxidation. The EE and CE were more toxic than the EAE on Caco‑2 cells with IC50 values – 26.45 ± 3.57 and 36.36 ± 6.87 μg/mL, respectively. At high dose, all fractions were not toxic to human’s PBMCs, but they slightly induced RBC hemolysis by 3-6 percent.Conclusion: The DS grown in Southern Thailand had antioxidant properties, toxic to Caco‑2 cells and not toxic to normal cells. High dose and continuous consumption of the DS must be concerned. Further safety assessment models including animals and humans should be performed to find safety dose of the DS.

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    Nooin, R., Pitchakarn, P., Kanchai, C., & Jaikang, C. (2019). Assessments of Antioxidant, Antilipid Peroxidation, and In-vitro Safety of Derris scandens Vine Extracts from Southern Thailand. Pharmacognosy Research, 11(1), 60-66. https://doi.org/10.4103/pr.pr_141_18

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    © 2019 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0