Pharmacognosy Research logo
Submit
Pharmacognosy ResearchACADEMIC PUBLISHING
Article

Cytotoxic Compounds from Wrightia pubescens (R.Br.)

1, 2, 3, 4, 3

  1. 1Biology Department, De La Salle University Laguna Campus, Biñan City, Laguna 4024, PHILIPPINES., 2Biology Department, De La Salle University 2401 Taft Avenue,Manila 0922, PHILIPPINES.
  2. 2Biology Department, De La Salle University 2401 Taft Avenue,Manila 0922, PHILIPPINES., 3Center for Natural Science and Environmental Research, De La Salle University 2401 Taft Avenue, Manila 0922, PHILIPPINES.
  3. 3Chemistry Department, De La Salle University 2401 Taft Avenue, Manila 0922, PHILIPPINES.
  4. 4National Research Institute of Chinese Medicine, Ministry of Health and Welfare 155-1, Li-Nong St., Sec. 2, Taipei 112, TAIWAN.

CORRESPONDENCE

Mariquit M. De Los Reyes

Biology Department, De La Salle University Laguna Campus, Biñan City, Laguna 4024, PHILIPPINES.

mariquit.delosreyes@dlsu.edu.ph

Volume 10, Issue 1 · pp. 9-15 · 2018 · DOI: 10.4103/pr.pr_45_17

ABSTRACT

Background: Mixtures of ursolic acid (1) and oleanolic acid (2) (1:1 and 1:2), oleanolic acid (2), squalene (3), chlorophyll a (4), wrightiadione (5), and α‑amyrin acetate (6) were isolated from the dichloromethane (CH2Cl2) extracts of the leaves and twigs of Wrightia pubescens (R.Br.).Objectives: To test for the cytotoxicity potentials of 1–6.Materials and Methods: The antiproliferative activities of 1–6 against three human cancer cell lines, breast (MCF‑7) and colon (HT‑29 and HCT‑116), and a normal cell line, human dermal fibroblast neonatal (HDFn), were evaluated using the PrestoBlue® cell viability assay.Results: Compounds 4, 1 and 2 (1:2), 2, 1 and 2 (1:1), and 5 exhibited the most cytotoxic effects against HT‑29 with half maximal inhibitory concentration (IC50) values of 0.68, 0.74, 0.89, 1.70, and 4.07 μg/mL, respectively. Comparing 2 with its 1:1 mixture with 1 (IC50 = 1.70 and 7.18 μg/mL for HT‑29 and HCT‑116, respectively) and 1:2 mixture with 1 (0.74 and 3.46 μg/mL for HT‑29 and HCT‑116, respectively), 2 also showed strong cytotoxic potential against HT‑29 and HCT‑116 (0.89 and 2.33 μg/mL, respectively). Unlike the mixtures which exhibited low effects on MCF‑7 (IC50 = 20.75 and 30.06 μg/mL for 1:1 and 1:2, respectively), 2 showed moderate activity against MCF‑7 (10.99 μg/mL). Compound 6 showed the highest cytotoxicity against HCT‑116 (IC50 = 4.07 μg/mL).Conclusion: Mixtures of 1 and 2 (1:1 and 1:2), 2, 3, 4, 5, and 6 from the CH2Cl2 extracts of the leaves and twigs of W. pubescens (R.Br.) exhibited varying cytotoxic activities. All the compounds except 6 exhibited the strongest cytotoxic effects against HT‑29. On the other hand, 6 was most cytotoxic against HCT‑116. Overall, the toxicities of 1–6 were highest against HT‑29, followed by HCT‑116 and MCF‑7. All the compounds showed varying activities against HDFn (IC50 <30 μg/mL).

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Reyes, M. M. D. L., Oyong, G. G., Ng, V. A. S., Shen, C., & Ragasa, C. Y. (2018). Cytotoxic Compounds from Wrightia pubescens (R.Br.). Pharmacognosy Research, 10(1), 9-15. https://doi.org/10.4103/pr.pr_45_17

    Copyright and licence

    © 2018 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0