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Protection of CCl4-Induced Liver and Kidney Damage by Phenolic Compounds in Leaf Extracts of Cnestis ferruginea (de Candolle)

1★, 2, 2★ Corresponding author

  1. 1Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria
  2. 2Pharmacology Unit, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan

CORRESPONDENCE

Adisa A. Rahmat

Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria

radisa22@yahoo.com

Volume 6, Issue 1 · pp. 19-28 · 2013 · DOI: 10.4103/0974-8490.122913

ABSTRACT

The chemoprevention of chemically-induced hepatotoxicity is a crucial means of minimizing susceptibility to hepatic carcinogenesis and plants remain a rich source of anti-hepatotoxicants with antioxidant properties. The protective role of defatted-methanol (MECF) and ethyl acetate fractions (EF), obtained from Leaves of Cnestis ferruginea in rats induced with carbon tetrachloride (CCl4) toxicity was investigated. Adult male Wistar rats were orally administered MECF or EF (125 – 500 mg/kg bwt/5mL) or silymarin (25 mg/kg bwt/5 mL) separately for three days before intervention with an intraperitoneal dose of CCl4. Biomarkers of liver and kidney toxicity as well as Ca2+ regulation were evaluated. Pre-treatment with MECF and EF significantly (P < 0.05) decreased the activities of serum alanine and aspartate aminotransferases, levels of urea, creatinine and cholesterol. A significantly (P < 0.05) enhanced Ca2+-ATPase activity and lowered levels of membrane cholesterol: Phospholipid ratio were observed in liver microsomes of pre-treated as compared to CCl4-only treated rats. Rat liver superoxide dismutase activity was enhanced by 125 mg/kg and 250 mg/kg of EF and MECF, while decreases were observed at 500 mg/kg. MECF and EF, like silymarin, attenuated CCl4-induced hepatotoxicity, microsomal membrane Ca2+-ATPase inactivation and renal dysfunction. Phytochemistry of MECF revealed the presence of anthraquinones, cardiac and flavone glycosides, tannins and trihydroxyl phenol. These findings suggest that the mechanism of hepatoprotection elicited by MECF and EF, involve its antioxidative properties and regulation of Ca2+ homeostasis.

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    Rahmat, A. A., Dar, F. A., & Choudhary, I. M. (2013). Protection of CCl4-Induced Liver and Kidney Damage by Phenolic Compounds in Leaf Extracts of Cnestis ferruginea (de Candolle). Pharmacognosy Research, 6(1), 19-28. https://doi.org/10.4103/0974-8490.122913

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    © 2013 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0