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Extraction, gas chromatography–mass spectrometry analysis and screening of fruits of Terminalia chebula Retz. for its antimicrobial potential

1★, 1★ Corresponding author

  1. 1Department of Botany, Laboratory of Plant Tissue Culture and Secondary Metabolites, University of Rajasthan, Rajasthan, India

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Geeta Singh

Department of Botany, Laboratory of Plant Tissue Culture and Secondary Metabolites, University of Rajasthan, Rajasthan, India

geetsingh600@gmail.com

Volume 5, Issue 3 · pp. 162-168 · 2013 · DOI: 10.4103/0974-8490.112421

ABSTRACT

Terminalia chebula is called the “king of medicines” in Tibet and is always listed first in the Ayurvedic meteria medica because of its extraordinary powers of healing. Identification, isolation and screening of pyrogallol which are responsible for antimicrobial property of fruits of Terminalia chebula. Ethyl acetate fraction of fruits of Terminalia chebula was subjected to Gas chromatography–mass spectrometry (GC-MS) for the components present in the extract. Sixty four constituents were identified out of which kaempferol-3-O-rutinoside flavonoid and Vitamin E has been detected for the first time in fruits of this plant. Pyrogallol (46.26%) which was the major component of the extract in GC-MS analysis was isolated and screened for antimicrobial activity against selected test pathogens by Disc Diffusion Assay. Crude ethyl acetate fraction of the fruits was showing the same activity potential as was observed for pure pyrogallol which was the major component as per GC-MS analysis. The most sensitive species among the bacteria was Enterobacter aerogenes with highest inhibition zone (IZ = 31 mm; AI = 1.409 ± 0.046) even at minimum inhibitory concentration (0.039 mg/ml). Hence activity shown by crude ethyl acetate fraction might be due to pyrogallol present in the extract. On the basis of results it can be advocate that achieved crude ethyl acetate fraction can be explored for preparing antimicrobial drugs in future for the infectious caused by the pathogens tested in the study.

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    Singh, G., & Kumar, P. (2013). Extraction, gas chromatography–mass spectrometry analysis and screening of fruits of Terminalia chebula Retz. for its antimicrobial potential. Pharmacognosy Research, 5(3), 162-168. https://doi.org/10.4103/0974-8490.112421

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    © 2013 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0