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Validation of High‑Performance Liquid Chromatography Method for Quantification of Costunolide Content in Laurus nobilis

1, 2, 2, 1, 2

  1. 1Analytical Research and Development, INDIA.
  2. 2Phytochemistry Lab, Vidya Herbs Pvt. Ltd, Bangalore, Karnataka, INDIA.

CORRESPONDENCE

Puttaswamy Naveen

Analytical Research and Development, INDIA.

analytical@vidyaherbs.com

Volume 10, Issue 4 · pp. 422-425 · 2018 · DOI: 10.4103/pr.pr_31_18

ABSTRACT

Objective: To establish a method to determine the content of costunolide in Laurus nobilis leaves by high‑performance liquid chromatography (HPLC).Materials and Methods: The separation was performed on a reversed‑phase C18 column (100 Å, 150 mm × 4.6 mm, 5 μm pore size) using a mobile phase composed of water:acetonitrile (40:60) at a flow rate of 1.0 ml/min. The detection was carried out on a ultraviolet detector at 210 nm. The developed method was validated according to the requirements for International Conference on Harmonisation guidelines.Results: The proposed method for costunolide was validated for linearity with excellent correlation coefficient (r2 > 0.999). The relative standard deviation (RSD) is less than 1% in precision (i.e repeatability and intermediate) of the method. The recovery rate for costunolide was within 100.54%–102.62%. The limit of detection and limit of quantitation were 2.29 and 6.64 parts per million, respectively.Conclusion: The developed HPLC method is simple, rapid, precisely, accurately, and widely accepted and it is recommended for efficient assays in routine work.

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    Naveen, P., Lingaraju, H. B., Chandrappa, S., Priya, K. S., & Prasad, K. S. (2018). Validation of High‑Performance Liquid Chromatography Method for Quantification of Costunolide Content in Laurus nobilis. Pharmacognosy Research, 10(4), 422-425. https://doi.org/10.4103/pr.pr_31_18

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    © 2018 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0