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  Indian J Med Microbiol
 

Figure 3: Lactoferrin inhibited the activation of nuclear transcription factor-kappa B in interleukin-1β-stimulated human osteoarthritis chondrocytes. Primary chondrocytes (70.80% confluent) were pretreated with lactoferrin (L) (25–75 μg/ml) and Bay 11–7082 (10 μM) for 2 h and stimulated by interleukin-1β (10 ng/ml) for 30 min. Activated nuclear factor-kappa B p65 in the nucleus was determined by highly specific transcription factor ELISA kit (Abcam). The positive control nuclear extract supplied with the kit was used. Data are representative of three experiments and is presented as mean ± standard error of mean #P < 0.05 versus untreated chondrocytes; #P < 0.05 versus L or Bay 11–7082 treated chondrocytes

Figure 3: Lactoferrin inhibited the activation of nuclear transcription factor-kappa B in interleukin-1β-stimulated human osteoarthritis chondrocytes. Primary chondrocytes (70.80% confluent) were pretreated with lactoferrin (L) (25–75 μg/ml) and Bay 11–7082 (10 μM) for 2 h and stimulated by interleukin-1β (10 ng/ml) for 30 min. Activated nuclear factor-kappa B p65 in the nucleus was determined by highly specific transcription factor ELISA kit (Abcam). The positive control nuclear extract supplied with the kit was used. Data are representative of three experiments and is presented as mean ± standard error of mean #<i>P</i> < 0.05 versus untreated chondrocytes; #<i>P</i> < 0.05 versus L or Bay 11–7082 treated chondrocytes