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  Indian J Med Microbiol
 

Figure 2: Chromatogram obtained by HPLC. Chromatograms of the hydroalcoholic extracts of P. ovatum obtained from roots (a), leaves (b) and stems (c) Piper ovatum; where the piperovatine (1), piperlonguminine (2). (d) Chromatogram of the standard piperlonguminine (RT = 24.46 min.). Chromatographic conditions: Metasil ODS column; mobile phase: acetonitrile: water 0% of acetonitrile for 60 % (0-30 min.) and acetonitrile: water 60:40 (v/v) (30-40 min.) with 1% acetic acid; flow rate: 1.0mL/min; room temperature; detection: 280 nm (at full page width)

Figure 2: Chromatogram obtained by HPLC. Chromatograms of the hydroalcoholic extracts of P. ovatum obtained from roots (a), leaves (b) and stems (c) Piper ovatum; where the piperovatine (1), piperlonguminine (2). (d) Chromatogram of the standard piperlonguminine (RT = 24.46 min.). Chromatographic conditions: Metasil ODS column; mobile phase: acetonitrile: water 0% of acetonitrile for 60 % (0-30 min.) and acetonitrile: water 60:40 (v/v) (30-40 min.) with 1% acetic acid; flow rate: 1.0mL/min; room temperature; detection: 280 nm (at full page width)