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  Indian J Med Microbiol
 

Figure 1(a) : Photomicrograph of MDA-MB-231 cell lines after 6 h treatment with 0.3% v/v DMSO (control). Original magnification is x 400 viewed under bright field using fluorescent microscope (Nikon eclopse 80 i). The control (0.3% w/v DMSO) did not cause the changes in the morphology and integrity of MDA-MB-231 cells. Figure 1(b) : Photomicrograph of MDA-MB-231 cell lines after 6 h treatment with 20 g/mL of ethylacetate extract from G. asiatica roots. Original magnification is ~ 400 viewed under bright field using fluorescent microscope (Nikon eclopse 80 i). The ethylacetate extract from G. asiatica roots at 20 g/mL caused the MDA-MB-231 cells to detach from the surface and the cells become rounded. Figure 1(c) : Photomicrograph of MDA-MB-231 cell lines stained with annexin-V substrate after 6 h treatment with 20 g/mL of ethylacetate extract from G. asiatica roots. Original magnification is x 400. The cells were stained with fluorescently labeled sulforhodamine 101 annexin-V and the cells were viewed under Texas-red filter (510-560 nm) using fluorescent microscope (Nikon eclopse 80 i). Red border around the MDA-MB-231 cells indicating phosphatidylserine translocation from inner to the outer leaflet of the cell membrane. Figure 1(d) : Photomicrograph of MDA-MB-231 cell lines stained with caspases-3 substrate after 6 h treatment with 20 g/mL of ethylacetate extract from G. asiatica roots. Original magnification is x 400. The cells were stained with fluorescently labeled Nuc ViewTM 488 caspase-3 substrate and the cells were viewed under Fluorescein isothiocyanate (FITC, 450-490 nm) using fluorescent microscope (Nikon eclopse 80 i). Green nucleus in the MDA-MB-231 cells indicating caspase-3 activation. Figure 1(e) : Merged photomicrograph of stained MDA-MB-231 cell lines after 6 h treatment with 20 g/mL of ethylacetate extract from G. asiatica roots. Green nucleus surrounded by red border indicating the apoptoic MDA-MB-231 cells.

Figure 1(a) : Photomicrograph of MDA-MB-231 cell lines after 6 h treatment with 0.3% v/v DMSO (control). Original magnification is x 400 viewed under bright field using fluorescent microscope (Nikon eclopse 80 i). The control (0.3% w/v DMSO) did not cause the changes in the morphology and integrity of MDA-MB-231 cells.
Figure 1(b) : Photomicrograph of MDA-MB-231 cell lines after 6 h treatment with 20 g/mL of ethylacetate extract from G. asiatica roots. Original magnification is ~ 400 viewed under bright field using fluorescent microscope (Nikon eclopse 80 i). The ethylacetate extract from G. asiatica roots at 20 g/mL caused the MDA-MB-231 cells to detach from the surface and the cells become rounded.
Figure 1(c) : Photomicrograph of MDA-MB-231 cell lines stained with annexin-V substrate after 6 h treatment with 20 g/mL of ethylacetate extract from G. asiatica roots. Original magnification is x 400. The cells were stained with fluorescently labeled sulforhodamine 101 annexin-V and the cells were viewed under Texas-red filter (510-560 nm) using fluorescent microscope (Nikon eclopse 80 i). Red border around the MDA-MB-231 cells indicating phosphatidylserine translocation from inner to the outer leaflet of the cell membrane.
Figure 1(d) : Photomicrograph of MDA-MB-231 cell lines stained with caspases-3 substrate after 6 h treatment with 20 g/mL of ethylacetate extract from G. asiatica roots. Original magnification is x 400. The cells were stained with fluorescently labeled Nuc ViewTM 488 caspase-3 substrate and the cells were viewed under Fluorescein isothiocyanate (FITC, 450-490 nm) using fluorescent microscope (Nikon eclopse 80 i). Green nucleus in the MDA-MB-231 cells indicating caspase-3 activation.
Figure 1(e) : Merged photomicrograph of stained MDA-MB-231 cell lines after 6 h treatment with 20 g/mL of ethylacetate extract from G. asiatica roots. Green nucleus surrounded by red border indicating the apoptoic MDA-MB-231 cells.