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2011| January-March | Volume 3 | Issue 1
Online since
April 7, 2011
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ORIGINAL ARTICLES
Standardization of some herbal antidiabetic drugs in polyherbal formulation
Harinarayan Singh Chandel, AK Pathak, Mukul Tailang
January-March 2011, 3(1):49-56
DOI
:10.4103/0974-8490.79116
PMID
:21731396
Background:
Ayurvedic formulations are used to treat a wide variety of diseases including diabetes mellitus Standardization of herbal formulation is essential in order to assess the quality of drugs. The present paper reports standardization of eight herbal anti-diabetic drugs−
Momordica charantia
(seeds),
Syzigium cumini
(seeds),
Trigonella foenum
(seeds),
Azadirachta indica
(leaves),
Emblica offi cinalis
(fruits),
Curcuma longa
(rhizomes),
Gymnema sylvestre
(leaves),
Pterocarpus marsupium
(heart-wood) individually and in polyherbal marketed samples of Baidyanath Madhumehari Churna
Material and Methods:
Shivayu Madhuhari Churna, Meghdut Madhushoonya Churna and were compared to the in-house preparation for physicochemical properties.
Results and Conclusions:
The limits obtained from the different physicochemical parameters of the individual eight herbal drugs and the marketed formulations could be used as reference standard for standardization of the anti-diabetic drugs in a quality control laboratory.
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4
Colon-targeted quercetin delivery using natural polymer to enhance its bioavailability
Anil Singhal, H Jain, Vipin Singhal, Edwin J Elias, Ahmad Showkat
January-March 2011, 3(1):35-39
DOI
:10.4103/0974-8490.79113
PMID
:21731393
The aim of the present study is to develop a polymer (Guar Gum)-based matrix tablet (using quercetin as a model drug) with sufficient mechanical strength, and promising
in vitro
mouth-to-colon release profile. By definition, an oral colonic delivery system should retard drug release in the stomach and small intestine, and allow complete release in the colon. By drug delivery to the colon would therefore ensure direct treatment at the disease site, lower dosing, and fewer systemic side effects. Quercetin is antioxidant in nature and used to treat colon cancer, but they have poor absorption in the upper part of the gastrointestinal tract (GIT). As a site for drug delivery, the colon offers a near neutral pH, reduced digestive enzymatic activity, a long transit time, and an increased responsiveness to absorption enhancers. By achieving a colon-targeted drug delivery system, the absorption of quercetin may be increased, which leads to better bioactivity in fewer doses.
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6,304
79
15
Hepatoprotective activity of
Ocimum sanctum
alcoholic leaf extract against paracetamol-induced liver damage in Albino rats
Kingshuk Lahon, Swarnamoni Das
January-March 2011, 3(1):13-18
DOI
:10.4103/0974-8490.79110
PMID
:21731390
Background:
There is a lack of reliable hepatoprotective drugs in modern medicine to prevent and treat drug-induced liver damage. Leaves of Sacred/Holy Basil, i.e. Green Tulsi (
Ocimum sanctum), belonging to family Lamiaceae are used traditionally for their hepatoprotective effect. We wanted to evaluate the hepatoprotective activity of
Ocimum sanctum and observe whether synergistic hepatoprotection exists with silymarin.
Materials and
Methods:
Albino rats (150-200 g) were divided into five groups. Groups A and B were normal and experimental controls, respectively. Groups C, D and E received the alcoholic extract of
Ocimum Sanctum
leaves (OSE) 200 mg/kg BW/day, silymarin 100 mg/kg BW/day and OSE 100 mg/kg BW/day + silymarin 50 mg/kg BW/day p.o., respectively, for 10 days. Hepatotoxicity was induced in Groups B, C, D and E on the eighth day with paracetamol 2 g/kg BW/day. The hepatoprotective effect was evaluated by performing an assay of the serum proteins, albumin globulin ratio, alkaline phosphatase, transaminases and liver histopathology. The assay results were presented as mean and standard error of mean (SEM) for each group. The study group was compared with the control group by one-way ANOVA, followed by Bonferoni's test. A
P
-value of <0.01 was considered significant.
Results:
In groups C, D and E, liver enzymes and albumin globulin ratio were significantly (
P
< 0.01) closer to normal than in group B. Reduction in sinusoidal congestion, cloudy swelling and fatty changes and regenerative areas of the liver were observed on histopathological examination in groups C, D and E, whereas group B showed only hepatic necrosis.
Conclusion:
The Ocimum sanctum alcoholic leaf extract shows significant hepatoprotective activity and synergism with silymarin.
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13
In vitro
antiproliferative activity of
Annona reticulata
roots on human cancer cell lines
HM Suresh, B Shivakumar, K Hemalatha, SS Heroor, DS Hugar, K.R.S Sambasiva Rao
January-March 2011, 3(1):9-12
DOI
:10.4103/0974-8490.79109
PMID
:21731389
Background:
The phytochemical and pharmacological activities of
Annona reticulata
components suggest a wide range of clinical application in lieu of cancer chemotherapy.
Materials and Methods:
Ethanol and aqueous extracts of roots of
Annona reticulata
Linn were studied for their
in vitro
antiproliferative activity on
A-549
(human lung carcinoma),
K-562
(human chronic myelogenous leukemia bone marrow)
, HeLa
(human cervix) and
MDA-MB
(human adenocarcinoma mammary gland) cancer cell lines by MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay.
Results:
The ethanol extract exhibited a prominent inhibitory effect against
A-549, K-562, HeLa
and
MDA-MB
human cancer cell lines at a concentration range between 10 and 40 μg/ml, whereas the aqueous extract showed a lower activity at the same concentration. Simultaneously, the effect of the ethanol extract toward the inhibition of
Vero
cell line proliferation was lower in comparison with the cancer cell lines.
Conclusion:
The significant antiproliferative activity of the ethanol extract of
Annona reticulata
roots against
A-549, K-562, HeLa
and
MDA-MB
human cancer cell lines may be attributed toward the collective presence of acetogenins, alkaloids and lower inhibitory effect on
Vero
cell line, which suggests
Annona reticulata
be used as a chemopreventive agent in cancer therapy.
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4,166
58
10
Anti-ulcerogenic and in vitro antioxidant activities of
Lagenaria breviflora
(LB) whole fruit ethanolic extract in laboratory animals
SA Onasanwo, Neetu Singh, AB Saba, AA Oyagbemi, OA Oridupa, Gautam Palit
January-March 2011, 3(1):2-8
DOI
:10.4103/0974-8490.79108
PMID
:21731388
Background:
The present study was undertaken to evaluate the anti-ulcer and antioxidant activities of the ethanol extract of
Lagenaria breviflora
(EELB) whole fruit in laboratory rats.
Methods:
The anti-ulcer property of the ethanolic extract of the whole fruit of
Lagenaria breviflora
(LB) was assessed using the cold-restraint stress-induced (CRU) gastric ulcer, pyloric ligation-induced (PL) gastric ulcer, aspirin-induced (ASP) gastric ulcer and alcohol-induced (AL) gastric ulcer models. The scavenging activity of the LB extract was examined with 1, 1-diphenyl-2-picryl-hydrazyl (DPPH), Nitric oxide, Hydroxyl radical and Superoxide anion scavenging models.
Results:
EELB (50, 100, 150 and 200 mg/kg, b.w.) protected against the CRU gastric ulcer dose dependently. Similarly, 150 mg/kg b.w. of the LB extract protected against the PL gastric ulcer, ASP gastric ulcer and AL gastric ulcer and was comparable to omeprazole (10 mg/kg b.w.) or Suscralfate (500 mg/kg b.w.), respectively. The
in vitro
antioxidant activity of LB was demonstrated by its ability to quench free radicals generated by nitric oxide and superoxide anion with a concomitant scavenging potential against DPPH-induced radical formation.
Conclusion:
Taken together, the study showed that the whole fruit extract possess potent anti-ulcer and antioxidant activities.
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3,949
46
9
Antihyperglycemic effect of the alcoholic seed extract of
Swietenia macrophylla
on streptozotocin-diabetic rats
Kalpana Kalaivanan, Kodukkur Viswanathan Pugalendi
January-March 2011, 3(1):67-71
DOI
:10.4103/0974-8490.79119
PMID
:21731399
Background:
Streptozotocin (STZ) selectively destroys the pancreatic insulin secreting cells, leaving less active cells and resulting in a diabetic state. The present study was designed to investigate the antihyperglycemic effect of the ethanolic seed extract of
Swietenia macrophylla
(SME) in normal and STZ-diabetic rats.
Materials and Methods:
The experimental groups were rendered diabetic by intraperitoneal injection of a single dose of STZ (40 mg/kg body weight [BW]). Rats with glucose levels > 200 mg/dL were considered diabetic and were divided into 5 groups. Three groups of diabetic animals were orally administered, daily with seed extract at a dosage of 50, 100, and 200 mg/kg BW. One group of STZ rats was treated as diabetic control and the other group was orally administered 600 ΅g/kg BW glibenclamide daily.
Results:
Graded doses of seed extract and glibenclamide showed a significant reduction in blood glucose levels and improvement in serum insulin levels. The extract also improved body weight and promoted liver glycogen content. After treatment, hemoglobin (Hb) level increased and glycosylated Hb level significantly decreased in diabetic rats. The activities of the carbohydrate metabolic enzymes showed significant changes in the rats. Of the 3 doses, 100 mg dose showed maximum activity. Histological investigations of pancreas also supported the biochemical findings.
Conclusions:
Thus, our findings indicate the folklore use of the seed for diabetes and the mechanism seems to be insulin secretion.
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3,715
52
12
Effect of methanolic extract of
Lagenaria siceraria
(Molina) Standley fruits on marble-burying behavior in mice: Implications for obsessive-compulsive disorder
RP Prajapati, MV Kalaria, VP Karkare, SK Parmar, NR Sheth
January-March 2011, 3(1):62-66
DOI
:10.4103/0974-8490.79118
PMID
:21731398
Context:
Obsessive-compulsive disorder (OCD) is the fourth most common mental disorder and is diagnosed nearly as often as asthma and diabetes mellitus. Over the last decade, the inhibition of burying of glass marbles by mice has been used as an index of anti-OCD drug action in the so-called marble-burying test.
Lagenaria siceraria
(Molina) Standley (LS), commonly known as "bottle gourd" (English), possesses several medicinal properties; little is known about its action as a nerve tonic.
Objective:
The purpose of this study was to characterize the anti-OCD (anti-compulsive) activity of the methanolic extract of the fruits of
L. siceraria
(Molina) Standley (LS) using the marble-burying behavior in mice.
Materials and Methods:
The experiment was conducted in specific animal models of Swiss albino mice to evaluate marble-burying behavior.
Results and Conclusions:
Intraperitonial administration of 25 and 50 mg/kg of LS extract significantly (
P
< 0.001) decreased the total number of buried marbles. The effect was comparable to that of the fluoxetine (10 mg/kg, i.p.). Fluoxetine and LS fruit extract do not produce any overt motor dysfunction. Preliminary phytochemical screening of the methanolic extract of LS showed the presence of flavonoids, saponins, sterols, proteins, tannins and carbohydrates. The results of the study for the first time show that the plant possesses anti-compulsive (anti-OCD) activity, confirming the traditional claims. Future research should focus on the identification and the mechanism of action of the constituents from this plant.
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3,695
58
4
Mining of simple sequence repeats in the Genome of
Gentianaceae
R Sathishkumar, P T.V Lakshmi, A Annamalai, V Arunachalam
January-March 2011, 3(1):19-29
DOI
:10.4103/0974-8490.79111
PMID
:21731391
Simple sequence repeats (SSRs) or short tandem repeats are short repeat motifs that show high level of length polymorphism due to insertion or deletion mutations of one or more repeat types. Here, we present the detection and abundance of microsatellites or SSRs in nucleotide sequences of
Gentianaceae
family. A total of 545 SSRs were mined in 4698 nucleotide sequences downloaded from the National Center for Biotechnology Information (NCBI). Among the SSR sequences, the frequency of repeat type was about 429 -mono repeats, 99 -di repeats, 15 -tri repeats, and 2 --hexa repeats. Mononucleotide repeats were found to be abundant repeat types, about 78%, followed by dinucleotide repeats (18.16%) among the SSR sequences. An attempt was made to design primer pairs for 545 identified SSRs but these were found only for 169 sequences.
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3,596
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Analgesic activity and acute toxicity study of
Semecarpus anacardium
stem bark extracts using mice
GM Lingaraju, H Joy Hoskeri, V Krishna, P Suresh Babu
January-March 2011, 3(1):57-61
DOI
:10.4103/0974-8490.79117
PMID
:21731397
Background:
The analgesic activity of petroleum ether, chloroform and methanol extracts of
Semecarpus anacardium
was investigated by tail flicking and writhing method using acetyl salicylic acid as the standard reference.
Materials and Methods:
The staircase method was adopted for the determination of the acute toxicity. LD
50
of the petroleum ether extract and the chloroform extract was 700 mg/kg; however, the LD
50
for the methanol extract was 500 mg/kg. After 1 h of oral administration of the extracts, 0.6% acetic acid was administered intraperitoneally and the analgesic activity was evaluated.
Results:
The number of writhing observed in the control group was 73.33 writhes. The methanol extract showed a significant analgesic activity, with 28.33 writhes, than the petroleum ether extract and the chloroform extract. But, all the extracts showed proved to be less potent than the standard drug which showed 2.33 writhes. Animals pretreated with saline did not show a signify cant effect on the latent period of tail-flick response. The analgesic effect of the petroleum ether extract was comparatively less evident. The maximum possible analgesia (MPA) increased up to 9.1% which remained elevated above the basal levels throughout the observation period. The MPA calculated for the chloroform extract increased to 14.03%. However, the analgesic effect of the methanol extract was also observed at 0.5 h following oral administration and the effect remained significant throughout the 3 h observation period, and was increased to 20.43%.
Conclusion:
Consistent analgesic activity of all the three
S. anacardium
extracts was observed by both the methods. The methanol extract was more potent than the petroleum ether and chloroform extracts but was less effective than the standard drug. This investigation supported the ethnomedicinal claims of
S. anacardium
.
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2
Evaluation of acute toxicity and hepatoprotective activity of the methanolic extract of
Dichrostachys cinerea
(Wight and Arn.) leaves
P Suresh Babu, V Krishna, KR Maruthi, K Shankarmurthy, Ramesh K Babu
January-March 2011, 3(1):40-43
DOI
:10.4103/0974-8490.79114
PMID
:21731394
Background:
D. cinerea
are the chief source of drug compounds that are active against various ailments such as jaundice, inflammations rheumatism, fever, asthma, body ache, chest problems, toothache, ulcers, wounds, eye diseases and have an aphrodisiac property. In present study, It was aimed to test the hepatoprotective activity of the plant.
Material and Methods:
The methanolic extract of
Dichrostachys cinerea
(Mimoseae) leaves was subjected to evaluation of acute toxicity and hepatoprotective property, using albino mice and rats. The parameters for estimation of liver function, based on serum markers such as total bilirubin, total protein, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase with histopathological profile of the liver tissue, were studied consequently.
Results:
The methanolic extract did not show any mortality up to a dose of 3500 mg/kg body weight. The methanolic extract showed significant hepatoprotectivity. The histopathological profile of the drug-treated liver tissue demonstrated similar morphology as that of controls.
Conclusions:
Methanolic extract of Dichrostachys cinerea was found to have significant hepatoprotective activity.
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3,246
46
2
The study of aqueous extract of
Ficus religiosa
Linn. on cytokine TNF-α in type 2 diabetic rats
H Kirana, MV Jali, BP Srinivasan
January-March 2011, 3(1):30-34
DOI
:10.4103/0974-8490.79112
PMID
:21731392
Background:
Chronic systemic inflammation is an early process in pathogenesis of type 2 diabetes. Hence the present study was aimed to investigate the effect of traditionally known plant
Ficus religiosa
on elevated glucose and inflammatory marker namely tumor necrosis factor (TNF)-α in type 2 diabetic rats.
Methods:
Type 2 diabetes was induced by administering streptozotocin (90 mg/kg, i.p.) in neonatal rat model. Aqueous extract of
F. religiosa
at a dose of 100 and 200 mg/kg was given orally to desired group of animals for a period of 4 weeks. After 4 weeks of drug treatment, parameters such as fasting blood glucose, postprandial blood glucose and TNF-α in serum were analyzed.
Results:
Aqueous extract of
F. religiosa
at both dose levels i.e., 100 and 200 mg/kg decreased the elevated glucose and TNF-α in type 2 diabetic rats. The extract at 200 mg/kg had more pronounced effect.
Conclusion:
Modulation of cytokine TNF-α by the aqueous extract of
F. religiosa
indicates that the anti-inflammatory and immunomodulatory property of the plant is related with its potential anti-diabetic activity.
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2,952
46
6
Partial
in vitro
and
in vivo
red scorpion venom neutralization activity of
Andrographis paniculata
Ranjana I Brahmane, Swanand S Pathak, Vikrant V Wanmali, Kartik J Salwe, S John Premendran, Bhawna B Shinde
January-March 2011, 3(1):44-48
DOI
:10.4103/0974-8490.79115
PMID
:21731395
Objective:
Red scorpion (
Mesobuthus tamulus
) is the most lethal among all poisonous species of scorpions. Envenoming by
Mesobuthus tamulus
is quite common along the western coast of India, without any established therapy.
Andrographis paniculata
is one of the plants that has long been used in traditional herbal medicine for the treatment of poisoning by animal bites. Hence, the study was planned to evaluate the ethanolic extract of
Andrographis paniculata
for the treatment of
Mesobuthus tamulus
envenoming.
Materials and Methods:
Ethanolic extract of the plant
Andrographis paniculata
was obtained using a soxhelet apparatus. Lyophilized venom sample of
Mesobuthus tamulus
was used. Swiss albino mice weighing 20-30 g were used in the study. Calculation of LD
99
of
Mesobuthus tamulus
venom was performed using Turner's method. Acute toxicity of
Mesobuthus tamulus
venom and its neutralization by the plant extract at a dose of 1 g/kg and 2 g/kg
in vivo
was seen. Neutralization of the lethal venom effect of
Mesobuthus tamulus
by plant extract at the dose of 1 g/kg and 2 g/kg by Alam and Gome's method (
in vitro
) was also seen.
Results:
The LD
99
of
Mesobuthus tamulus
venom from this study was determined to be 25.12 ΅g/g and the LD
50
was 15.85 ΅g/g. In the acute toxicity and
in vivo
neutralization study, plant extract at the dose of 1 g/kg and 2 g/kg resulted in a mean survival of 62.667 min and 39.333 min, respectively. Neutralization of the lethal venom effect of
Mesobuthus tamulus
by the plant extract at the dose of 1 g/kg and 2 g/kg by Alam and Gome's method (
in vitro
) showed a mean survival of 49.667 min and 42.5 min, respectively.
Conclusion:
The ethanolic extract of
Adrographis paniculata
has some protective effect against the red scorpion venom in mice but does not offer any survival benefit.
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2,585
49
5
EDITORIAL
Prophylactic measures
Ambrose Furey
January-March 2011, 3(1):1-1
DOI
:10.4103/0974-8490.79107
PMID
:21731387
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1,877
46
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BOOK REVIEW
Biomedical Research from Ideation to Publication
Arun H.S Kumar
January-March 2011, 3(1):72-72
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1,396
25
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