Validation of high-performance liquid chromatography method for quantification of costunolide content in <i>Laurus nobilis</i> :Puttaswamy Naveen, Harakanahalli Basavegowda Lingaraju, Siddappa Chandrappa, Koretekera Shekharappa Priya, Kodimule Shyam Prasad, Pharmacognosy Research

ORIGINAL ARTICLE
Year : 2018 | Volume : 10 | Issue : 4 | Page : 422--425

Validation of high-performance liquid chromatography method for quantification of costunolide content in Laurus nobilis

Puttaswamy Naveen¹, Harakanahalli Basavegowda Lingaraju², Siddappa Chandrappa², Koretekera Shekharappa Priya¹, Kodimule Shyam Prasad²
¹ Analytical Research and Development, Vidya Herbs Pvt. Ltd, Bangalore, Karnataka, India
² Phytochemistry Lab, Vidya Herbs Pvt. Ltd, Bangalore, Karnataka, India

Correspondence Address:
Dr. Puttaswamy Naveen
Analytical Research and Development, Vidya Herbs Pvt. Ltd, Jigani, Anekal Taluk, Bengaluru, Karnataka
India

Objective: To establish a method to determine the content of costunolide in Laurus nobilis leaves by high-performance liquid chromatography (HPLC). Materials and Methods: The separation was performed on a reversed-phase C18 column (100 Å, 150 mm × 4.6 mm, 5 μm pore size) using a mobile phase composed of water:acetonitrile (40:60) at a flow rate of 1.0 ml/min. The detection was carried out on a ultraviolet detector at 210 nm. The developed method was validated according to the requirements for International Conference on Harmonisation guidelines. Results: The proposed method for costunolide was validated for linearity with excellent correlation coefficient (r² > 0.999). The relative standard deviation (RSD) is less than 1% in precision (i.e repeatability and intermediate) of the method. The recovery rate for costunolide was within 100.54%–102.62%. The limit of detection and limit of quantitation were 2.29 and 6.64 parts per million, respectively. Conclusion: The developed HPLC method is simple, rapid, precisely, accurately, and widely accepted and it is recommended for efficient assays in routine work. Abbreviation Used: HPLC: High-performance liquid chromatography; UV: Ultraviolet; ICH: International Conference on Harmonisation; r²: Correlation coefficient; WHO: World Health Organization; ppm: Parts per million; % RSD: Percentage relative standard deviation

How to cite this article:
Naveen P, Lingaraju HB, Chandrappa S, Priya KS, Prasad KS. Validation of high-performance liquid chromatography method for quantification of costunolide content in Laurus nobilis.Phcog Res 2018;10:422-425

How to cite this URL:
Naveen P, Lingaraju HB, Chandrappa S, Priya KS, Prasad KS. Validation of high-performance liquid chromatography method for quantification of costunolide content in Laurus nobilis. Phcog Res [serial online] 2018 [cited 2021 Mar 1 ];10:422-425
Available from: http://www.phcogres.com/article.asp?issn=0974-8490;year=2018;volume=10;issue=4;spage=422;epage=425;aulast=Naveen;type=0