Pharmacognosy Research

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 9  |  Issue : 5  |  Page : 53--56

Picroside I and picroside II from tissue cultures of Picrorhiza kurroa


Yamjala Ganeshkumar, Ajmera Ramarao, Ciddi Veeresham 
 Department of Pharmacognosy, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana, India

Correspondence Address:
Ciddi Veeresham
University College of Pharmaceutical Sciences, Kakatiya University, Warangal - 506 009, Telangana
India

Background: Picrorhiza kurroa (PK) belongs to Scrophulariaceae family and is a representative endemic, medicinal herb, widely distributed throughout the higher altitudes of alpine Himalayas from west to east, between 3000 and 4500 m above mean sea level. Objective: The objective of the present study is to assess the production of picroside I and picroside II from tissue cultures of PK. Materials and Methods: Auxiliary shoot tips of PK were incubated in Murashige and Skoog medium supplemented with indole-3-butyric acid and kinetin phytohormones. The callus produced was collected at different time intervals and was processed for extraction of picroside I and picroside II followed by thin layer chromatography and high-performance liquid chromatography HPLC analysis. Results: The maximum growth index was found to be 5.109 ± 0.159 at 16-week-old callus culture. The estimation of picroside-I and picroside-II was carried out by (HPLC) analysis; quantity of secondary metabolite found to be 16.37 ± 0.0007 mg/g for PK-I and 6.34 ± 0.0012 mg/g for PK-II. Conclusion: This is the first attempt to produce the Picroside-I and II in large amount by the tissue culture technique. It can be observed that the method of callus culture can be used in production of secondary metabolites Picroside-I and II from PK


How to cite this article:
Ganeshkumar Y, Ramarao A, Veeresham C. Picroside I and picroside II from tissue cultures of Picrorhiza kurroa.Phcog Res 2017;9:53-56


How to cite this URL:
Ganeshkumar Y, Ramarao A, Veeresham C. Picroside I and picroside II from tissue cultures of Picrorhiza kurroa. Phcog Res [serial online] 2017 [cited 2021 Mar 6 ];9:53-56
Available from: http://www.phcogres.com/article.asp?issn=0974-8490;year=2017;volume=9;issue=5;spage=53;epage=56;aulast=Ganeshkumar;type=0