Pharmacognosy Research

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 9  |  Issue : 5  |  Page : 23--26

Antihyperglycemic potential of saponin-enriched fraction from Pithecellobium dulce Benth. seed extract


Mahesh Kumar, Jeyabalan Govindrajan, Narendra Kumar Nyola 
 Faculty of Pharmacy, Rajasthan University of Health Sciences, Jaipur; Department of Pharmacognosy, Alwar College of Pharmacy, Alwar, Rajasthan, India

Correspondence Address:
Mahesh Kumar
Alwar College of Pharmacy, Alwar, Rajasthan
India

Background: Indian traditional system of medicine uses Pithecellobium dulce for the treatment of diabetes mellitus. Objectives: This study aims to develop an extract rich in saponins derived from seeds of the plant and to evaluate its antihyperglycemic potential in vitro and in vivo. Materials and Methods: Defatted seeds were extracted with methanol and processed to afford saponin-enriched fraction (Pithecellobium dulce saponin-enriched fraction [PDSEF]). This fraction was evaluated for its potential to inhibit enzymes such as α-glucosidase and α-amylase, in vitro. The fraction was subjected to oral toxicity study followed by in vivo sucrose tolerance test. An analytical high-performance liquid chromatography method was developed for fingerprinting of the fraction. Results: The method adopted for enrichment of saponins was robust enough to enrich saponin content to 96.37% ±1.21% w/w. PDSEF displayed superior inhibition of enzymes (α-glucosidase and α-amylase with IC50of 5.12 ± 0.15 μg/ml and 17.28 ± 0.23 μg/ml, respectively) compared to acarbose. It was found to be safe in mice up to 2000 mg/kg and significantly prevented blood glucose level in sucrose tolerance test by inhibiting enzymes responsible for hydrolysis of sucrose. Conclusion: PDSEF displayed excellent antihyperglycemic activity in vitro and in vivo and should be evaluated further to develop it as a promising drug for the management of diabetes mellitus.


How to cite this article:
Kumar M, Govindrajan J, Nyola NK. Antihyperglycemic potential of saponin-enriched fraction from Pithecellobium dulce Benth. seed extract.Phcog Res 2017;9:23-26


How to cite this URL:
Kumar M, Govindrajan J, Nyola NK. Antihyperglycemic potential of saponin-enriched fraction from Pithecellobium dulce Benth. seed extract. Phcog Res [serial online] 2017 [cited 2021 Apr 14 ];9:23-26
Available from: http://www.phcogres.com/article.asp?issn=0974-8490;year=2017;volume=9;issue=5;spage=23;epage=26;aulast=Kumar;type=0