Pharmacognosy Research

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 9  |  Issue : 1  |  Page : 27--33

Chronic intake of green propolis negatively affecting the rat testis


Grasiela Dias de Campos Severi-Aguiar1, Suellen Josine Pinto2, Cristina Capucho1, Camila Andrea Oliveira3, Maria Aparecida Diamante1, Renata Barbieri2, Fabrícia Souza Predes4, Heidi Dolder1 
1 Reproductive Biology Laboratory, Department of Structural and Functional Biology, Biology Institute, State University of Campinas – UNICAMP, Campinas, Brazil
2 Heath Sciences Nucleus, Hermínio Ometto Univerity Center, UNIARARAS, Araras, São Paulo, Brazil
3 Graduate Program in Biomedical Sciences, Hermínio Ometto University Center, UNIARARAS, Araras, São Paulo, Brazil
4 Department of Biological Sciences, State University of Paraná (UNESPAR), Paranaguá, Paraná, Brazil

Correspondence Address:
Grasiela Dias de Campos Severi-Aguiar
Departamento de Biologia Estrutural e Funcional, Universidade Estadual de Campinas – UNICAMP, CP 6109, CEP 13083-863, Campinas, São Paulo
Brazil

Background: Human and animal evidence suggests that environmental toxicants may have an adverse impact on male reproductive health, reducing the population's reproductive output. Owing to the renewed attraction for natural products, some of them constitute effective alternatives to mitigate these effects. Propolis is a candidate for this use because of its intrinsic properties. In many situations, it improved the testicular damage and alleviated the toxic effects induced by environmental contaminant exposure. Objective: The aim of this study was to investigate possible alterations of testicular parameters and certify if its use is really advantageous to the testis, since this could affect rat reproductive function. Materials and Methods: Forty-eight adult male Wistar rats were divided into four groups (Co = control, T1 = 3 mg propolis/kg/day, T2 = 6 mg/kg/day, T3 = 10 mg/kg/day) and were exposed during 56 days. The testes were assessed with morphometrical, stereological, and ultrastructural analyses. Cell proliferation and death were diagnosed, respectively, by immunocytochemistry. Connexin 43 (Cx43) and N-cadherin transcript levels were determined by reverse transcription-polymerase chain reaction. Results: Increased cell proliferation and Leydig cell volume were observed in T2, and in contrast, Cx43 upregulation and cell death were observed in T3. Both T2 and T3 showed ultrastructural abnormalities in testicular parenchyma. Conclusion: We recommend a cautious intake of propolis to avoid deleterious effects.


How to cite this article:
Severi-Aguiar GD, Pinto SJ, Capucho C, Oliveira CA, Diamante MA, Barbieri R, Predes FS, Dolder H. Chronic intake of green propolis negatively affecting the rat testis.Phcog Res 2017;9:27-33


How to cite this URL:
Severi-Aguiar GD, Pinto SJ, Capucho C, Oliveira CA, Diamante MA, Barbieri R, Predes FS, Dolder H. Chronic intake of green propolis negatively affecting the rat testis. Phcog Res [serial online] 2017 [cited 2021 Jan 19 ];9:27-33
Available from: http://www.phcogres.com/article.asp?issn=0974-8490;year=2017;volume=9;issue=1;spage=27;epage=33;aulast=Severi-Aguiar;type=0