Pharmacognosy Research

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 8  |  Issue : 4  |  Page : 292--297

Inhibition of human cytochrome p450 2c8-catalyzed amodiaquine n-desethylation: Effect of five traditionally and commonly used herbs


Yasotha Devi Muthiah1, Chin Eng Ong2, Siti Amrah Sulaiman3, Rusli Ismail4 
1 Pharmacogenetics Research Group, Institute for Research in Molecular Medicine, Universiti Sains, Malaysia
2 School of Pharmacy, Monash University , 47500 Bandar Sunway, Selangor, Malaysia
3 Department of Pharmacology, School of Medical Sciences, Universiti Sains , 16150 Kubang Kerian, Kelantan, Malaysia
4 Centre of Excellence for Research in AIDS, Universiti Malaya, 59990 Kuala Lumpur, Malaysia

Correspondence Address:
Chin Eng Ong
School of Pharmacy, Monash University Malaysia, 47500 Bandar Sunway, Selangor
Malaysia

Background: In Southeast Asia and many parts of the world, herbal products are increasingly used in parallel with modern medicine. Objective: This study aimed to investigate the effects of herbs commonly used in Southeast Asia on activity of cytochrome P450 2C8 (CYP2C8), an important human hepatic enzyme in drug metabolism. Materials and Methods: The selected herbs, such as Eurycoma longifolia Jack (ELJ), Labisia pumila (LP), Echinacea purpurea (EP), Andrographis paniculata (AP), and Ginkgo biloba (GB), were subjected to inhibition studies using an in vitro CYP2C8 activity marker, amodiaquine N-desethylase assay. Inhibition parameters, inhibitory concentration 50% (IC50), and Kivalues were determined to study the potency and mode of inhibition. Results: All herbs inhibited CYP2C8 with the following order of potency: LP > ELJ > GB > AP > EP. LP and ELJ inhibited potently at Ki's of 2 and 4 times the Kiof quercetin, the positive control. The inhibition by LP was uncompetitive in nature as compared to competitive or mixed type inhibition observed with other herbs. GB exhibited moderate inhibitory effect at a Ki6 times larger than quercetin Ki. AP and EP, on the other hand, showed only weak inhibition. Conclusion: The herbs we chose represented the more commonly used herbs in Southeast Asia where collision of tradition and modernization in healthcare, if not properly managed, may lead to therapeutic misadventures. We conclude that concurrent consumption of some herbs, in particular, LP and ELJ, may have relevance in drug-herb interactions via CYP2C8 inhibition in vivo.


How to cite this article:
Muthiah YD, Ong CE, Sulaiman SA, Ismail R. Inhibition of human cytochrome p450 2c8-catalyzed amodiaquine n-desethylation: Effect of five traditionally and commonly used herbs.Phcog Res 2016;8:292-297


How to cite this URL:
Muthiah YD, Ong CE, Sulaiman SA, Ismail R. Inhibition of human cytochrome p450 2c8-catalyzed amodiaquine n-desethylation: Effect of five traditionally and commonly used herbs. Phcog Res [serial online] 2016 [cited 2021 Jan 21 ];8:292-297
Available from: http://www.phcogres.com/article.asp?issn=0974-8490;year=2016;volume=8;issue=4;spage=292;epage=297;aulast=Muthiah;type=0