Pharmacognosy Research

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 8  |  Issue : 1  |  Page : 50--55

Antioxidant and Immunomodulatory activity of Hydroalcoholic extract and its fractions of leaves of Ficus benghalensis Linn.


Anil Subhash Bhanwase, Kallanagouda Ramappa Alagawadi 
 Department of Pharmaceutical Chemistry, KLEU'S College of Pharmacy, Belgaum, Karnataka, India

Correspondence Address:
Anil Subhash Bhanwase
Department of Pharmaceutical Chemistry, KLEU«SQ»S College of Pharmacy, Belgaum - 10, Karnataka
India

Background: Ficus benghalensis is a folk medicine indigenous plant of India. Several studies on this plant reported and focused on the biological profile of the plant. Objectives: This study is aimed to evaluate the antioxidant and immunomodulatory activity of F. benghalensis leaf extract using various in vitro screening methods of both parameters. Materials and Methods: Hydroalcoholic (FB1) extract and it«SQ»s four fractions viz. n-hexane (FB2), n-butanol (FB3), chloroform (FB4), and water (FB5) of leaves of F. benghalensis investigated for their free radical scavenging activity using 1-1-diphneyl-2-picrylhydrazyl and 2, 2«SQ»-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radicals. A dose-response curve was plotted and IC 50 values were determined to assess antioxidant activity. Nitroblue tetrazolium test, phagocytosis of killed Candida albicans and candidacidal assay were carried out to assess the immunomodulatory activity. Positive non-lymphoid cell number, mean particle number of killed C. albicans, percent value of killed C. albicans by neutrophils were calculated and presented. Results: All extracts showed antioxidant and prominent immunomodulatory activity with compared to standard. Conclusions: Hydroalcoholic (FB1) extract and its four fractions viz. n-hexane (FB2), n-butanol (FB3), chloroform (FB4), and water (FB5) showed promising antioxidant and immunomodulatory activity.


How to cite this article:
Bhanwase AS, Alagawadi KR. Antioxidant and Immunomodulatory activity of Hydroalcoholic extract and its fractions of leaves of Ficus benghalensis Linn. Phcog Res 2016;8:50-55


How to cite this URL:
Bhanwase AS, Alagawadi KR. Antioxidant and Immunomodulatory activity of Hydroalcoholic extract and its fractions of leaves of Ficus benghalensis Linn. Phcog Res [serial online] 2016 [cited 2020 Nov 26 ];8:50-55
Available from: http://www.phcogres.com/article.asp?issn=0974-8490;year=2016;volume=8;issue=1;spage=50;epage=55;aulast=Bhanwase;type=0