Pharmacognosy Research

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 6  |  Issue : 1  |  Page : 19--28

Protection of CCl 4 -Induced Liver and Kidney Damage by Phenolic Compounds in Leaf Extracts of Cnestis ferruginea (de Candolle)


Adisa A Rahmat1, Farooq Ahsana Dar2, Iqbal M Choudhary2 
1 Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria
2 Pharmacology Unit, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan

Correspondence Address:
Adisa A Rahmat
Department of Biochemistry, College of Medicine of the University of Lagos, P.M.B. 12003, Lagos, Nigeria

Background: The chemoprevention of chemically-induced hepatotoxicity is a crucial means of minimizing susceptibility to hepatic carcinogenesis and plants remain a rich source of anti-hepatotoxicants with antioxidant properties. Objective: The protective role of defatted-methanol (MECF) and ethyl acetate fractions (EF), obtained from Leaves of Cnestis ferruginea in rats induced with carbon tetrachloride (CCl 4 ) toxicity was investigated. Materials and Methods: Adult male Wistar rats were orally administered MECF or EF (125 - 500 mg/kg bwt/5mL) or silymarin (25 mg/kg bwt/5 mL) separately for three days before intervention with an intraperitoneal dose of CCl 4. Biomarkers of liver and kidney toxicity as well as Ca 2+ regulation were evaluated. Results: Pre-treatment with MECF and EF significantly (P < 0.05) decreased the activities of serum alanine and aspartate aminotransferases, levels of urea, creatinine and cholesterol. A significantly (P < 0.05) enhanced Ca 2+ -ATPase activity and lowered levels of membrane cholesterol: Phospholipid ratio were observed in liver microsomes of pre-treated as compared to CCl 4 -only treated rats. Rat liver superoxide dismutase activity was enhanced by 125 mg/kg and 250 mg/kg of EF and MECF, while decreases were observed at 500 mg/kg. MECF and EF, like silymarin, attenuated CCl 4 -induced hepatotoxicity, microsomal membrane Ca 2+ -ATPase inactivation and renal dysfunction. Phytochemistry of MECF revealed the presence of anthraquinones, cardiac and flavone glycosides, tannins and trihydroxyl phenol. Conclusion: These findings suggest that the mechanism of hepatoprotection elicited by MECF and EF, involve its antioxidative properties and regulation of Ca 2+ homeostasis.


How to cite this article:
Rahmat AA, Dar FA, Choudhary IM. Protection of CCl 4 -Induced Liver and Kidney Damage by Phenolic Compounds in Leaf Extracts of Cnestis ferruginea (de Candolle).Phcog Res 2014;6:19-28


How to cite this URL:
Rahmat AA, Dar FA, Choudhary IM. Protection of CCl 4 -Induced Liver and Kidney Damage by Phenolic Compounds in Leaf Extracts of Cnestis ferruginea (de Candolle). Phcog Res [serial online] 2014 [cited 2021 Jan 20 ];6:19-28
Available from: http://www.phcogres.com/article.asp?issn=0974-8490;year=2014;volume=6;issue=1;spage=19;epage=28;aulast=Rahmat;type=0