Pharmacognosy Research

ORIGINAL ARTICLE
Year
: 2013  |  Volume : 5  |  Issue : 2  |  Page : 71--79

Immune-stimulatory and anti-inflammatory activities of Curcuma longa extract and its polysaccharide fraction


Chinampudur V Chandrasekaran, Kannan Sundarajan, Jothie R Edwin, Giligar M Gururaja, Deepak Mundkinajeddu, Amit Agarwal 
 R and D Centre, Natural Remedies Pvt. Ltd., Bangalore, India

Correspondence Address:
Chinampudur V Chandrasekaran
Plot No. 5B, Veerasandra Indl Area, 19th K.M. Stone, Hosur Road, Bangalore - 560 100, Karnataka
India

Background: While curcuminoids have been reported to possess diverse biological activities, the anti-inflammatory activity of polar extracts (devoid of curcuminoids) of Curcuma longa (C. longa) has seldom been studied. In this study, we have investigated immune-stimulatory and anti-inflammatory activities of an aqueous based extract of C. longa (NR-INF-02) and its fractions in presence and absence of mitogens. Materials and Methods: Effects of NR-INF-02 (Turmacin TM , Natural Remedies Pvt. Ltd., Bangalore, India) on proliferation, nitric oxide (NO), monocyte chemotactic protein-1 (MCP-1), interleukins (ILs) and prostaglandin (PGE 2 ) levels of mouse splenocytes and mouse macrophage (RAW264.7) cells were determined. Results: NR-INF-02 increased splenocytes number in presence and absence of lipopolysaccharide (LPS) or concanavalin A. Treatment of NR-INF-02 showed a significant increase of NO, IL-2, IL-6, IL-10, IL-12, interferon (IFN) gamma, tumor necrosis factor (TNF) alpha and MCP-1 production in unstimulated mouse splenocytes and mouse macrophages. Interestingly, NR-INF-02 showed potent inhibitory effect towards release of PGE 2 and IL-12 levels in LPS stimulated mouse splenocytes. Further, NR-INF-02 was fractionated into polysaccharide fraction (F1) and mother liquor (F2) to study their immune-modulatory effects. F1 was found to be more potent than F2 toward inhibiting PGE 2 and IL-12 in LPS stimulated splenocytes. Conclusion: Present findings revealed the novel anti-inflammatory property of NR-INF-02 and its polysaccharide fraction by inhibiting the secretion of IL-12 and PGE 2 in vitro.


How to cite this article:
Chandrasekaran CV, Sundarajan K, Edwin JR, Gururaja GM, Mundkinajeddu D, Agarwal A. Immune-stimulatory and anti-inflammatory activities of Curcuma longa extract and its polysaccharide fraction.Phcog Res 2013;5:71-79


How to cite this URL:
Chandrasekaran CV, Sundarajan K, Edwin JR, Gururaja GM, Mundkinajeddu D, Agarwal A. Immune-stimulatory and anti-inflammatory activities of Curcuma longa extract and its polysaccharide fraction. Phcog Res [serial online] 2013 [cited 2021 Jun 19 ];5:71-79
Available from: http://www.phcogres.com/article.asp?issn=0974-8490;year=2013;volume=5;issue=2;spage=71;epage=79;aulast=Chandrasekaran;type=0