Pharmacognosy Research

: 2010  |  Volume : 2  |  Issue : 1  |  Page : 41--44

Profile of heavy metals in some medicinal plants from Ghana commonly used as components of herbal formulations

Kofi Annan1, Asante Isaac Kojo2, Asare Cindy2, Asare-Nkansah Samuel3, Bayor Marcel Tunkumgnen4,  
1 Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of Ghana, Legon
2 Botany, University of Ghana, Legon
3 Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, University of Ghana, Legon
4 Pharmaceutics, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

Correspondence Address:
Kofi Annan
Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi Ghana


The levels of some heavy metals in 27 medicinal plant species from Ghana were studied in order to evaluate their health implications. These plant species, especially those used in the treatment of diseases such as hypertension, diabetes and asthma may require long term usage. The metals were copper, zinc, iron, manganese, nickel and cadmium. Atomic Absorption Spectrophotometry (wet digestion) was used for the analyses, and content of metals per sample was expressed as percent µg/g. Daily total intake of these metals is discussed based on the recommended daily intake of the medicinal plants or their corresponding formulations. From the results of the study zinc, copper and cadmium were present in all the plant species examined. Manganese was present in all species except V. amygdalina. Iron was found in all except five species (82%), whilst nickel was (rather rare) detected in only eight (30%) of the plant species. Significant variations in metal content existed (P<0.05) among the medicinal plant species with respect to the heavy metals evaluated. The concentrations of copper, zinc, cadmium and manganese were within their respective maximum permissible daily levels. However, some species, especially Ocimum canum (8), Clausena anisata and Rauwolfia vomitoria had levels of iron higher than the maximum permissible level of 1000 µg/day and may require care to avoid iron toxicity. The results also highlighted the differences in contents of minerals in Lippia multiflora obtained from different locations in Ghana. The findings generally suggest that the use of these plant species for the management of diseases will not cause heavy metal toxicity and may be beneficial to the users in cases of micronutrient deficiency, as these metals were found to be present in readily bioavailable form.

How to cite this article:
Annan K, Kojo AI, Cindy A, Samuel AN, Tunkumgnen BM. Profile of heavy metals in some medicinal plants from Ghana commonly used as components of herbal formulations.Phcog Res 2010;2:41-44

How to cite this URL:
Annan K, Kojo AI, Cindy A, Samuel AN, Tunkumgnen BM. Profile of heavy metals in some medicinal plants from Ghana commonly used as components of herbal formulations. Phcog Res [serial online] 2010 [cited 2021 Feb 28 ];2:41-44
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Medicinal plants play a major role in the health care sector of developing nations for the management of diseases. Thus herbal medicines have a prominent role to play in the pharmaceutical markets and health care sector of the 21 st century. [1] Heavy metals as micronutrients are important for the proper functioning of vital organs in the body. For example, iron is a component of hemoglobin and other compounds used in respiration. Heavy metals are widespread in soil as a result of geo-climatic conditions and environmental pollution. Therefore, their assimilation and accumulation in plants is obvious. Together with other pollutants, heavy metals are discharged into the environment through industrial activity, automobile exhaust, heavy-duty electric power generators, municipal wastes, refuse burning and pesticides used in agriculture. [2] Human beings, animals and plants take up these metals from the environment through air and water. Heavy metals have the tendency to accumulate in both plants and human organs.

Since plants and animals are essential sources of micronutrients for man, it becomes necessary to monitor the levels in biological materials that are required by man for both dietary and medicinal purposes. This is because deficiency or excess of micronutrients can be factors of disease generation.

Even though a lot of phytochemical and bioactivity studies have been carried out on a number of medicinal plants in Ghana, [3] not much has been reported on the heavy metal contents of these plants. This study therefore sought to establish the presence, quantity and prevalence of six heavy metals (Cu, Zn, Fe, Mn, Ni and Cd) in 27 medicinal plants commonly used for the treatment, prevention and management of diseases in Ghana.

In view of the fact that micronutrients can be good, toxic or lethal depending on the dose, the study also evaluated the health implications of the heavy metals quantified, based on the recommended daily intake of medicinal plant decoctions. [4] The use of atomic absorption spectrophotometry for determination of the amounts of heavy metals in both organic and inorganic samples has been well reported. [5]

 Materials and Methods

Plant materials

A total of 27 different medicinal plant species used in the study were collected from their natural habitat and authenticated at the Department of Botany, University of Ghana, where voucher specimen of each plant species is deposited [Table 1]. The leaves were air-dried for four days and later pulverized.

Mineral analysis

An acid digest of each plant species was prepared by oxidizing 0.2 g of the powdered plant sample with a nitric/perchloric acid (2:1) mixture (10 ml). Aliquots of the mixture were used to estimate Fe, Cu, Zn, Mn, Ni and Cd by Analyst 400 Atomic Absorption Spectrometer. Each sample was analyzed thrice, and the data were reported as mean ± SD in µg/g.

Statistical analysis

The data were based on three replicates and subjected to analysis of variance. Standard errors of each individual nutrient of the samples were computed, and variations among the species were evaluated by least significance difference (LSD) at 5% level of probability (P = 0.05). Data analysis was conducted using the SAS Statistical Computer Package.

 Results and Discussion

From the study, zinc, copper and cadmium were present in all plant species examined. Manganese was present in all species except V. amygdalina. Iron was found in all except five species (82%), while nickel was (rather rare) detected in only eight of the plant species (30%) [Table 2].

The iron content found in all the plant species examined was generally relatively high (20-753 µg/g), with the three highest concentrations found in O. canum 8, C. anisata and R. vomitoria, respectively. The maximum permissible level (MPL) of iron is 1000 µg/day. [6] In this study, however, majority of the plant species examined (60%) had iron content above the maximum permissible level per 10 g daily dose of plant material or its equivalent formulation. Iron salts have an astringent action resulting in irritation of the gastrointestinal mucosa, which gives rise to gastric discomfort, nausea, vomiting and diarrhea or constipation. [7],[8] Therefore, the presence of these symptoms commonly associated with the intake of some medicinal plants may be due to iron toxicity.

Zinc content ranged from 43.5 µg/g in B. diffusa to 495.0 µg/g in C. anisata. Zinc is a component of many metalloenzymes, especially some enzymes which play a central role in nucleic acid metabolism. [9] Zinc is also a membrane stabilizer and a stimulator of the immune response. [10] Manifestations of acute zinc poisoning include nausea, vomiting, diarrhea, fever and lethargy. [7] The estimated safe and adequate daily intake of zinc is between 10,000 and 20,000 µg/day. [6] The zinc levels per 10 g of plant species were not more than 4950 µg, hence lower than the maximum permissible level and regarded as safe.

Manganese content was disproportionately very high in only a few species, including R. vomitoria (1455 µg/g) followed by G. sylvestre (1190 µg/g), A. cordifolia (645 µg/g) and V. africana (556 µg/g). The estimated safe and adequate daily dietary intake of manganese in adults is 11,000 µg/ day. [11] Therefore, most of the plant species examined (93%) can be said to have acceptable manganese content. Deficiency of manganese in humans may lead to immunodeficiency disorder, rheumatic arthritis in adults, disorder of bony cartilaginous growth in infants, as well as myocardial infarction and other cardiovascular diseases. [12]

Copper content was variable among the plant species, ranging from 8.0 to 114.5 µg/g, with G. sylvestre recording the highest amount. Copper is essential to the human body since it forms a component of many enzyme systems, such as cytochrome oxidase, lysyl oxidase and ceruloplasmin, an iron-oxidizing enzyme in blood. The observation of anemia in copper deficiency may probably be related to its role in facilitating iron absorption and in the incorporation of iron in hemoglobin. [6] However, copper could be toxic depending on the dose and duration of exposure. [7] The maximum permissible level (MPL) of copper is 12,000 µg/ day. [6] Therefore, the suggested average intake of about 10 g of plant material or its equivalent formulation gives a maximum of 1145 µg of copper per day. [4] This implies that the 27 medicinal plants evaluated contained safe levels of copper.

The MPL of nickel is 100 µg/day. [10] Although nickel was present in only eight species, all except V. rosea had nickel content higher than the permissible level. This however constitutes only 26%; the majority (74%) had nickel content within acceptable limits. Nickel exerts a potent toxic effect on peripheral tissues and on the reproductive system. [10] It also causes dose-related decreases in bone marrow cellularity and in granulocytomacrophage and pluripotent stem cell proliferative responses.

Cadmium was also found in all the plant species, and its concentration ranged from 22.5 µg/g in L. multiflora (Kadjebi) to 59.0 µg/g in L. multiflora (Kasoa). Cadmium is a nonessential trace element with uncertain direct functions in both plants and humans. [13] It is however reported that the lowest level of cadmium which can cause yield reduction in plants is 5 to 30 µg/g, and the maximum acceptable concentration for foodstuff is about 1 µg/g. [14] The results of this study indicated that about 80% of the plant species had cadmium content above 30 µg/g, which is essential for improved yield.

The overall results indicated clearly that heavy metals are present in Ghanaian medicinal plants and that the contents of these metals except iron were within acceptable and safe limits [Table 3]. Therefore, herbal formulations of these plant species can also be beneficial sources of appropriate and essential trace elements, though care must be taken to avoid iron toxicity, especially in higher doses.


Most of the plant species contained safe levels of the heavy metals analyzed and hence may have no adverse effects normally associated with heavy metal toxicity on people who patronize these products for their health needs.


We would like to thank the technical staff of the Ecological Laboratory, University of Ghana, Legon for their assistance in this work.


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