TY - JOUR T1 - Molecular Identification and Next‑Generation Sequence Analysis of Interspecies Genetic Variations among Three Varieties of Datura JF - Pharmacognosy Research Y1 - 2020 A1 - Goli Penchala Prasad A1 - Goli Penchala Pratap A1 - Srinivasan Marimuthu A1 - Shyam Baboo Prasad A1 - Gajendra Rao A1 - Anupam Kumar Mangal A1 - Naryanam Srikanth KW - Ayurveda KW - Datura KW - Internal transcribed spacer 4 KW - Mat k KW - Medicinal plants KW - Next-generation sequencing KW - Ribulose bisphosphate carboxylase/oxygenase form I gene AB -

Background: Datura is a well‑known plant used in Ayurveda. It is a widely growing plant from Solanaceae family, attributed to poisonous and medicinal values. It is used in the treatment of various skin diseases, fever, etc., It is also used for external application and rarely for oral administration. Objectives: The present study aims to compare three different varieties of Datura which include the species of Datura innoxia Mill., Datura metel L. and Datura arborea are used to study molecular marker and phylogenetic analysis. Materials and Methods: Whole‑genomic DNA was isolated from the leaves of Datura and the polymerase chain reactions amplification of DNA barcoding markers are rbcl, mat k, and internal transcribed spacer‑4 and 5 were analyzed by 0.8% agarose gel electrophoresis. Results: The DNA barcoding markers and next‑generation sequencing are able to identify the interspecies genetic variations among these closely related plant varieties of Datura. Conclusion: The interspecies genetic variations among these closely related three species of D. innoxia, metel, and arborea was closely related with Datura stramonium isolate NN003 chloroplast genome similarity of 98%, 99%, and 99%, respectively.

VL - 12 IS - 2 ER -