03055nas a2200277 4500008004100000245013600041210006900177260001600246300001200262490000700274520218800281653001702469653000902486653001402495653002402509653002402533653002002557653001702577653002102594653001802615100002002633700002402653700001702677700002002694856006302714 2020 eng d00aA Phytochemical Analysis and In vivo Effects of an Herbal Aphrodisiac Newtonia hildebrandtii on Male Wistar Rat Reproductive System0 aPhytochemical Analysis and In vivo Effects of an Herbal Aphrodis cAugust 2020 a243-2490 v123 a

Background: The study was aimed at screening phytochemicals and evaluating the effects of aqueous and 50% (v/v) methanol stem bark extracts of Newtonia hildebrandtii on male rat reproductive system. Materials and Methods: Thirty male Wistar rats were divided into five groups and were given different treatments of N. hildebrandtii extracts at 200 and 400 mg/kg body weight. After 8 weeks, body and right testis weight, sperm concentration, hormonal levels, and histological structure of the testis were evaluated. Calorimetric method was used for quantitative analysis of phenolic compounds, whereas gas chromatography–mass spectrometry (GC-MS) was used for fingerprinting of chemical compounds. Results: Testosterone, luteinizing hormone, estradiol, and follicle-stimulating hormone levels were significantly increased, whereas prolactin levels were significantly decreased in the treated groups compared to the control. Sperm parameters were significantly increased compared to the control. Treated animals showed significant increases in body weight, seminiferous tubule diameter, and germinal epithelial height with the testes showing all the stages of spermatogenesis. Phytochemical screening of the methanolic extracts revealed the presence of alkaloids, carbohydrates, reducing sugars, steroids, anthraquinones, flavonoids, phenols, and saponins. GC-MS analysis revealed the presence of dimethylsilanediol; (R, R)-2,3-butanediol; hexamethylcyclotrisiloxane; succinimide; 1H-1,2,3-triazole-4-carboxylic acid, 1-(4-amino-1,2,5-oxadiazol-3-yl)-5-ethyl-, ethyl ester; l-(+)-ascorbic acid 2,6-dihexadecanoate; cis-13,16 docasadienoic acid, methyl ester; 9-octadecenoic acid (Z)-, 2-hydroxy-1-(hydroxymethyl) ethyl ester; and 2-chloro-benzoic acid N'-[2-(3-oxo-3,4-dihydro-2H-benzo[1,4]thiazin-2-yl)-acetyl]-hydrazide. Conclusion: N. hildebrandtii appears to possess androgenic and anabolic effects. The aforementioned phytochemicals seem to be responsible for its aphrodisiac effects. This study supports the ethno-aphrodisiac claims that have been made against it.

10aAntioxidants10aDPPH10aEstradiol10aSeminiferous tubule10aSperm concentration10aSpermatogenesis10aTestosterone10aTotal flavonoids10aTotal phenols1 aMsiska, Thomson1 aMwakikunga, Anthony1 aTembo, David1 aLampiao, Fanuel uhttps://www.phcogres.com/article/2020/12/3/104103prpr11219