01768nas a2200193 4500008004100000245007300041210006900114260001700183300001200200490000600212520118700218653001601405653001601421653001401437653000901451100002501460700002201485856006701507 2010 eng d00aEvaluation of in vitro Cytotoxic Effect of Trichosanthes dioica Root0 aEvaluation of in vitro Cytotoxic Effect of Trichosanthes dioica  cJanuary 2011 a355-3580 v23 a

Background: Trichosanthes dioica Roxb. (Cucurbitaceae), called pointed gourd in English is a dioecious climber grown in India and used traditionally for various medicinal purposes. Methods: Present study was aimed to evaluate in vitro cytotoxic effect of dichloromethane (DCTD), methanol (METD), and aqueous (AQTD) extracts of T. dioica root using Allium cepa root meristems by keeping them in different concentrations of each test extract under specific experimental conditions followed by determination of root growth inhibition (root length and number) and mitotic index. Results: All the extracts significantly demonstrated concentration-dependent inhibition of root length and number and reduction in mitotic index, indicating antimitotic activity demonstrating cytotoxicity and genotoxicity. DCTD was found to be the most potent (EC 50 : 2.8 mg/ml), followed by METD and AQTD. Conclusion: The present study therefore, establishes promising in vitro cytotoxic and genotoxic property of T. dioica root against the test system.

10aAllium cepa10aAntimitotic10aCytotoxic10aRoot1 aBhattacharya, Sanjib1 aHaldar, Pallab, K uhttps://www.phcogres.com/article/2010/2/6/1041030974-849075454