02452nas a2200241 4500008004100000245007600041210006900117260001600186300001200202490000700214520169800221653000601919653003801925653002501963653001801988653001902006653001502025100002602040700003002066700002602096700002502122856006302147 2019 eng d00aPhytochemical Content and Antioxidant Activities of Pomelo Peel Extract0 aPhytochemical Content and Antioxidant Activities of Pomelo Peel  cAugust 2019 a244-2470 v113 a

Background: There is an increasing interest in the antioxidant measurement of some plant constituents. Plant component, especially fruit, has antioxidant components which are safe, economic, and powerful to reduce oxidative stress and replace synthetic ones. Objectives: To quantitatively measure the flavonoids, Vitamin C, and carotenoid content of hydroalcoholic extract and to estimate the antioxidant activity of pomelo peel extract (PPE) and minimum inhibitory concentration 50% (IC50) of the extract using 2,2‑diphenyl‑1‑picrylhydrazyl (DPPH) ethanolic solution. Materials and Methods: Hydroalcoholic PPE was prepared; total flavonoids using aluminum chloride method, Vitamin C using thiourea and 2,4‑dinitrophenylhydrazine method, and carotenoid content of the extract were estimated using 95% ethanol solution. All measurements were estimated using ultraviolet–visible spectrophotometer. Antioxidant activity and IC50 of PPE were measured using DPPH method. Results: Flavonoids, Vitamin C, and carotenoid content were measured as 21.20 mg/100 g, 15.13 mg/100 g, and 62.98 μg/g, respectively, of dry PPE. The DPPH radical scavenging activity exhibits a significant dose‑dependent inhibition of DPPH radical color, with IC50 being at a concentration of 68.55 μg/ml of the extract, comparable to 55.87 μg/ml IC50 of Vitamin C standard antioxidant. Conclusion: From the study results, we could conclude that pomelo peel possesses high antioxidant properties and it is potentially rich sources of natural antioxidants.

10a210a2‑diphenyl‑1‑picrylhydrazyl10aAntioxidant capacity10aAscorbic acid10aCitrus maxima.10aFlavonoids1 aAbudayeh, Zead, Helmi1 aKhalifa, Ihab, Ibrahim Al1 aMohammed, Shaimaa, M.1 aAhmad, Asser, Ashraf uhttps://www.phcogres.com/article/2019/11/3/104103prpr18018