02239nas a2200253 4500008004100000245012000041210006900161260001600230300001200246490000600258520138200264653001001646653003901656653004301695653001401738653001901752653001401771100002901785700002501814700002501839700002501864700002801889856006801917 2014 eng d00aAntiproliferative Effects of Plumbago rosea and its Purified Constituent Plumbagin on SK-MEL 28 Melanoma Cell Lines0 aAntiproliferative Effects of Plumbago rosea and its Purified Con cAugust,2014 a312-3190 v63 a

Background: Plumbago rosea is used in traditional systems of medicine for the preparation of formulations used for treating inflammations, cough, bronchitis, and gastrointestinal disorders, and also in conjunction with cancer chemotherapy. In the present study, the cytotoxic and anti-proliferative effects of plumbagin, and the ethanolic root extract of P. rosea (ETPR) was evaluated on SK-MEL 28 melanoma cell lines and human lymphocytes. Materials and Methods: MTT and apoptotic assays were used for the evaluation of cytotoxic and anti-proliferative effects, respectively. In addition, the effect of Plumbagin and ETPR in down regulation of BCL-2 expression is investigated using RT-PCR analysis. Results: Both plumbagin and ETPR dose-dependently decreased the cell viability more potently in melanoma cell lines. P. rosea extract demonstrated significant synergy in inhibiting BCL-2 expression than plumbagin. Moreover plumbagin showed more toxicity in human lymphocytes. Conclusion: Plumbagin has anti-cancer potential, but the side effects limits its use; yet plumbagin, in combination with other ingredients in Plumbago rosea extract, displays significant synergy leading to a stronger anticancer effect with significantly less toxicity.

10aBCL-210aethanolic root extract of P. rosea10aHigh performance liquid chromatography10aPlumbagin10aPlumbago rosea10aSK-MEL 281 aAnuf, Alexander, Ronaldo1 aRamachandran, Rajesh1 aKrishnasamy, Rajaram1 aGandhi, PS, Sudhakar1 aPeriyasamy, Sureshkumar uhttps://www.phcogres.com/article/2014/6/4/1041030974-8490138280