02780nas a2200301 4500008004100000245010800041210006900149260001400218300001200232490000700244520176400251653001902015653001402034653001102048653002102059653002602080100002802106700002902134700003302163700003002196700002802226700003602254700002702290700003702317700002902354700003302383856006202416 2021 eng d00aEmbryotoxicity of Yangambin Isolated from Ocotea duckei Vattimo-Gil in Gallus gallus domesticus Embryos0 aEmbryotoxicity of Yangambin Isolated from Ocotea duckei VattimoG cJune 2021 a135-1390 v133 a

Introduction: Yangambin, a lignan predominant in the leaves of Ocotea duckei Vattimo-Gil, has several biological activities, such as anti-convulsant, analgesic, anti-inflammatory and leishmanicidal. Objectives: The aim of this study was to evaluate the embryotoxicity of yangambin on the neurodevelopment of Gallus gallus domesticus embryos. Materials and Methods: 120 fertilized eggs were divided in three groups: G1 (PBS with 0.1 % Tween 80), G2 (50 μg/ml yangambin) and G3 (65 μg/ml yangambin) and each egg was inoculated with 100 μL of the respective solutions. The fertilized eggs were incubated at a temperature of 37.5°C, with a relative humidity of 65% to 75%, for 48 hr and then their embryos were histologically processed. Results: In staging, carried out according to Hamburger and Hamilton (1951), variations of stages were identified. In all groups, the morphological analysis revealed the closure of the anterior neuropore and absence of malformations in the optic vesicles and in the secondary encephalic vesicles. In the caudal region, a standard development of the neural tube was observed, with well-segmented somites and regression of the primitive line. The cross sections showed that the internal structure of the somite’s, composed of dermatome, myotome and sclerotome, was preserved. The statistical analysis did not show significant differences between the groups regarding the morphometry of the cephalic and caudal regions of the neural tube. Conclusion: Yangambin did not show embryotoxic effects on the neurodevelopment of Gallus gallus domesticus embryos, under the tested conditions.

10aChicken embryo10aLauraceae10aLignan10aNeurodevelopment10aSecondary metabolites1 ada Silva, Thiago, Felix1 aCastro, Maria, Isabel de1 ade Lucena, Marta, Gerusa Soa1 aSilva, Camilla, Vila Nova1 aPeixoto, Laisla, Rangel1 ade Carvalho, Eliane, Alves Band1 aPaz, Silvania, Tavares1 aGuimarães, Beathryz, Lethycya A1 aFilho, José, Maria Barb1 ada Silva, Eliete, Cavalcanti uhttps://www.phcogres.com/article/2021/13/3/105530pres1335