02452nas a2200253 4500008004100000245013300041210006900174260001600243300001200259490000600271520161000277653002501887653001401912653002801926653002701954653001201981100001901993700002902012700001702041700002502058700003002083700001702113856006802130 2014 eng d00aAntiallergic Effect of an Aqueous Leaf Extract of Pistia Stratiotes in Murine Model of Ovalbumin-induced Allergic Conjunctivitis0 aAntiallergic Effect of an Aqueous Leaf Extract of Pistia Stratio cAugust,2014 a274-2790 v63 a

Aim: The aim was to investigate the antiallergic effect of an aqueous leaf extract of Pistia stratiotes (ALPS) in a murine model of ovalbumin (OVA)-induced allergic conjunctivitis (AC). Materials and Methods: Prior to topical challenge (instillation of 1.5 mg OVA in 10 μL phosphate buffered saline into their conjunctival sacs) to induce AC, groups of sensitized Imprinting Control Region mice (injected IP, on day 1 and 7, with 0.2 ml solution of 100 μg OVA and 0.01 mg aluminum hydroxide in phosphate buffered saline), were treated with 5 mg/kg cetirizine, 10, 50 or 100 mg/kg of ALPS, or 2 ml/kg normal saline per os. Conjunctival redness, lid edema, tearing and lid scratching (clinical symptoms of AC) were scored. Serum OVA specific immunoglobulins were determined using ELISA. Histopathological assessment of the conjunctival mucosal tissue was conducted. The extract was screened for secondary plant metabolites. Results: Pretreatment with the extract significantly (P ≤ 0.05-0.01) and dose-dependently reduced the scores for clinical symptoms, which were marked in vehicle-pretreated mice. Pretreatment also lowered (P ≤ 0.01-0.001) serum OVA specific immunoglobulins. Mast cell infiltration and degranulation in conjunctival stroma (measured by an inflammatory score) in histopathological studies was also significantly low (P ≤ 0.05-0.01) on pretreatment. Conclusion: The ALPS exhibited interesting antiallergic activity and hence could be useful in managing AC.

10aConjunctival redness10aLid edema10aMast cell degranulation10aMast cell infiltration10aTearing1 aAbokyi, Samuel1 aKoffuor, George, Asumeng1 aKyei, Samuel1 aAsiamah, Emmanuel, A1 aAtobiga, Clement, Nsobire1 aAwuah, Agnes uhttps://www.phcogres.com/article/2014/6/4/1041030974-8490138243