02556nas a2200265 4500008004100000245010900041210006900150260001400219300001200233490000700245520165800252653002701910653003901937653001501976653002301991653001902014100002902033700003002062700002602092700003402118700002902152700001902181700002802200856006202228 2021 eng d00aAnticoagulant Activity of Crude and Phenolic Extracts of Dalbergia ecastaphyllum (L.) Taub. Dried Leaves0 aAnticoagulant Activity of Crude and Phenolic Extracts of Dalberg cJune 2021 a121-1280 v133 a

Background: Ischemic diseases are one of the leading causes of death worldwide; therefore, the search for new compounds with safer and more effective anticoagulant and antiplatelet action becomes relevant. Objectives: To evaluate the impact of Dalbergia ecastaphyllum (L.) Taub. dry leaf extract, a species of the Fabaceae family that is commonly found in Brazil, on blood coagulation. Materials and Methods: A statistical design of experiments was carried out with selection of five factors and two levels, resulting in 32 different ethanolic raw extracts. The extracts were resuspended in saline and tested in a pool of human blood. The activated partially thromboplastin time (APTT) and the prothrombin time (PT) were analysed in a Thrombolyzer®. Results: Treatment using ethanol 70%, a drug/solvent ratio of 5%, pH 9 and dynamic maceration for 6 hrs resulted in the best combination for the extraction of total phenols, while ethanol 70%, a drug/solvent ratio of 5% and dynamic maceration for 24 hrs showed the best flavonoid extraction, with the pH being inconclusive. On average, all extracts were capable of increasing both APTT and PT, especially those in lower dilutions (1:5); however, the overall efficacy of the extracts is inconclusive. Conclusion: The species under study showed important results in the blood clotting process in vitro, increasing the mean time of APTT and PT, which suggests that Dalbergia ecastaphyllum (L.) Taub. has some potential as a possible drug in the anticoagulant class.

10aAnticoagulant activity10aDalbergia ecastaphyllum (L.) Taub.10aFlavonoids10aPhenolic compounds10aPlant extracts1 aRovetta, Enrico, Rogatto1 aMagenta, Mara, Angelina G1 aGascon, Thaís, Moura1 aFonseca, Fernando, Luiz Affon1 ada Gama, Robson, Miranda1 aArmando, José1 aMoya, Horácio, Dorigan uhttps://www.phcogres.com/article/2021/13/3/105530pres1333