02517nas a2200217 4500008004100000245014500041210006900186260001500255300001200270490000600282520174500288653002202033653001902055653001702074653004802091100002402139700002202163700002002185700002602205856006802231 2014 eng d00aBiphasic effect of Solanum nigrum fruit aqueous extract on vascular mesenteric beds in non-diabetic and streptozotocin-induced diabetic rats0 aBiphasic effect of Solanum nigrum fruit aqueous extract on vascu cMarch,2014 a148-1520 v63 a

Background: In Asia, Solanum nigrum fruit is traditionally used to manage, control, and treat diabetes. Objective: This study was carried out to investigate the endothelium and nitric oxide roles in Solanum nigrum-induced vasorelaxation in non-diabetic and diabetic rat vessels. Materials and Methods: Diabetes was induced by a single i.p. injection of streptozotocin. Eight weeks later, superior mesenteric arteries of non-diabetic and diabetic groups were isolated and perfused according to the McGregor method. Solanum nigrum fruit extract (SNE) at concentrations of 0.00001 to 0.6 mg/ml was added to the medium and perfusion pressure was recorded. Results: Baseline perfusion pressure of diabetic group was significantly higher than non-diabetic rats in both intact and denuded endothelium. The low concentrations of SNE have vasodilatory effect in the diabetic and non-diabetic, but high concentrations of SNE produce initial significant contractions, followed by secondary relaxations in normal and diabetic rats. We observed vasorelaxation at low and high concentrations of SNE in both diabetic and non-diabetic groups after endothelium denudation. SNE-induced vasorelaxation in diabetic group is mediated by both endothelium and smooth muscle, but the relaxatory effect of SNE in non-diabetic group is not mediated by endothelium, and SNE has direct action on the smooth muscle. Conclusion: Although the part of SNE-induced relaxation in diabetic vessel was mediated by endothelium, nitric oxide didn't play any role in this action, and maybe we can use SNE in the management of diabetes vessel complications in future.

10aDiabetes mellitus10aMesenteric bed10aNitric oxide10aSolanum nigrum fruit extract vasorelaxation1 aSohrabipour, Shahla1 aKharazmi, Fatemah1 aSoltani, Nepton1 aKamalinejad, Mohammad uhttps://www.phcogres.com/article/2014/6/2/1041030974-8490129036