02792nas a2200241 4500008004100000245016500041210006900206260001500275300001200290490000600302520194500308653001802253653004202271653001302313653002502326100002302351700002402374700001902398700002102417700001802438700002602456856006802482 2014 eng d00aPresence of monoterpene synthase in four Labiatae species and Solid-Phase Microextraction- Gas chromatography-Mass Spectroscopy analysis of their aroma profiles0 aPresence of monoterpene synthase in four Labiatae species and So cMarch,2014 a138-1420 v63 a

Background: The family Lamiaceae (Labiatae) has included some medicinal plants. some monoterpene synthases, including linalool and limonene synthases, have been cloned and functionally characterized from several plants of Labiatae family. Materials and Methods: In this study, presence of linalool and limonene synthases, in four species of Labiatae family including Nepeta cataria, Lavandula angustifolia, Hyssopus officinalis and Salvia sclarea has been determined by molecular biological techniques together with the Head space SPME - GC-MS analysis of the aroma profile of these species. Results: Indicated that none of the plant species produced distinguishable bands with primer pairs related to d-limonene synthase. Distinguishable bands around 1800 bp in cDNA samples of L. angustifolia, H. officinalis and S. sclarea were observed regarding to the presence of linalool synthase. Head space SPME-GC-MS analysis of the aroma profiles of the above-mentioned plants showed that linalool (31.0%), linalyl acetate (18.2%), were found as the major compounds of L. angustifolia, while geraniol (5.5%), nerol (34.0%) and α- citral (52.0%) were identified as the main compounds of the N. cataria. The major components of H. officinalis and S. sclarea oils were determined as cis-pinocamphone (57.3%), and linalool (19.0%), linalyl acetate (51.5%), respectively. Conclusion: H. officinalis was rich of cyclic monoterpenes, L. angustifolia, N. cataria and S. sclarea showed considerable amount of linear monoterpenes. The aroma profile of the above-mentioned plants contained low concentration of sesquiterpenes except N. cataria, which indicated no sesquiterpene. The profiles of the main components of these plants are in agreement with molecular assays.

10aEssential oil10aHeadspace solid phase microextraction10aLabiatae10aMonoterpene synthase1 aSaeidnia, Soodabeh1 aGohari, Ahmad, Reza1 aHaddadi, Azita1 aAmin, Gholamreza1 aNikan, Marjan1 aHadjiakhoondi, Abbass uhttps://www.phcogres.com/article/2014/6/2/1041030974-8490129033