02062nas a2200253 4500008004100000245010200041210006900143260001500212300001200227490000600239520132800245653001801573653000901591653001701600653002201617653000901639653000701648653001701655100002001672700001401692700001501706700001901721856006801740 2014 eng d00aEffect on eNOS/NO Pathway in MIRI Rats with Preconditioning of GFPC from Dang Gui Si Ni Decoction0 aEffect on eNOSNO Pathway in MIRI Rats with Preconditioning of GF cMarch,2014 a133-1370 v63 a

Objective: In order to discover whether the eNOS/NO (endothelial nitric oxide synthase/nitric oxide) pathway is involved in the protective mechanisms of ischemic myocardium of DGSND (Dang Gui Si Ni Decoction) in MIRI (myocardial ischemia-reperfusion injury) SD rats. Materials and Methods: We made I/R (ischemia-reperfusion) model by ligating the left anterior-descending branch of the coronary artery (LAD) for 30 min and releasing the ligature for 120 min. eNOS (nitric oxide synthase) mRNA (message ribonucleic acid) and iNOS (inducible nitric oxide synthase) mRNA were measured by the methods of real-time RT-PCR (Real time Polychainase Chain Reaction), peNOS (phosphorylated eNOS) and iNOS protein were measured by the means of western blot. Results: In PPC group, real-time RT-PCR and western-blot analysis showed that eNOS mRNA and peNOS protein increased markedly (P < 0.05); iNOS mRNA and protein decreased significantly (P < 0.05). Conclusion: These results indicate that ischemic preconditioning (IPC) of GFPC from DGSND plays a protective role in I/R heart through regulating the eNOS/NO signal pathway, which could increase the eNOS gene expression and decrease the expression of iNOS mRNA.

10aCinnamic acid10aeNOS10aFerulic acid10aGlycyrrhizic acid10aMIRI10aNO10aPaeoniflorin1 aQian, Guo-qiang1 aPeng, Xia1 aCai, Chuan1 aZhao, Guo-ping uhttps://www.phcogres.com/article/2014/6/2/1041030974-8490129032