02633nas a2200217 4500008004100000245013200041210006900173260001800242300001000260490000600270520187600276653002702152653003402179653002302213653002102236653002102257100002102278700002402299700002402323856006802347 2014 eng d00aProtection of CCl 4 -Induced Liver and Kidney Damage by Phenolic Compounds in Leaf Extracts of Cnestis ferruginea (de Candolle)0 aProtection of CCl 4 Induced Liver and Kidney Damage by Phenolic  cDecember,2013 a19-280 v63 a

Background: The chemoprevention of chemically-induced hepatotoxicity is a crucial means of minimizing susceptibility to hepatic carcinogenesis and plants remain a rich source of anti-hepatotoxicants with antioxidant properties. Objective: The protective role of defatted-methanol (MECF) and ethyl acetate fractions (EF), obtained from Leaves of Cnestis ferruginea in rats induced with carbon tetrachloride (CCl 4 ) toxicity was investigated. Materials and Methods: Adult male Wistar rats were orally administered MECF or EF (125 - 500 mg/kg bwt/5mL) or silymarin (25 mg/kg bwt/5 mL) separately for three days before intervention with an intraperitoneal dose of CCl 4. Biomarkers of liver and kidney toxicity as well as Ca 2+ regulation were evaluated. Results: Pre-treatment with MECF and EF significantly (P < 0.05) decreased the activities of serum alanine and aspartate aminotransferases, levels of urea, creatinine and cholesterol. A significantly (P < 0.05) enhanced Ca 2+ -ATPase activity and lowered levels of membrane cholesterol: Phospholipid ratio were observed in liver microsomes of pre-treated as compared to CCl 4 -only treated rats. Rat liver superoxide dismutase activity was enhanced by 125 mg/kg and 250 mg/kg of EF and MECF, while decreases were observed at 500 mg/kg. MECF and EF, like silymarin, attenuated CCl 4 -induced hepatotoxicity, microsomal membrane Ca 2+ -ATPase inactivation and renal dysfunction. Phytochemistry of MECF revealed the presence of anthraquinones, cardiac and flavone glycosides, tannins and trihydroxyl phenol. Conclusion: These findings suggest that the mechanism of hepatoprotection elicited by MECF and EF, involve its antioxidative properties and regulation of Ca 2+ homeostasis.

10aCa 2+ -ATPase activity10aCCl 4 -induced hepatotoxicity10aCnestis ferruginea10aHepatoprotection10aRenal protection1 aRahmat, Adisa, A1 aDar, Farooq, Ahsana1 aChoudhary, Iqbal, M uhttps://www.phcogres.com/article/2014/6/1/1041030974-8490122913