02744nas a2200253 4500008004100000245013800041210006900179260001800248300000900266490000600275520190600281653002202187653002102209653002302230653002802253653001402281100002602295700002802321700001802349700002102367700001702388700001702405856006802422 2014 eng d00aComparative Study to Evaluate the Anti-viral Efficacy of Glycyrrhiza glabra Extract and Ribavirin Against the Newcastle Disease Virus0 aComparative Study to Evaluate the Antiviral Efficacy of Glycyrrh cDecember,2013 a6-110 v63 a

Background: The Newcastle disease represents as one of the most infectious viral disease, which afflicts almost every species of the birds. The causative agent of the disease is a single-stranded RNA virus with rapid replication capability. Objective: This study was performed to evaluate the comparative anti-viral efficacy and toxicity of Glycyrrhiza glabra aqueous extract and ribavirin against the Newcastle disease virus. Materials and Methods: The embryonated eggs were divided into six groups (A, B, C, D, E and F). Groups A, B, C, and D were further subdivided into three subgroups. The virus was identified by hemagglutination inhibition test. Spot hemagglutination test and viability of embryos were also evaluated. Three different concentrations i-e., 30 mg/100 ml, 60 mg/100 ml, and 120 mg/100 ml of the Glycyrrhiza aqueous extract and 10 μg/ml, 20 μg/ml, and 40 μg/ml ribavirin in deionized water were evaluated for their toxicity and anti-viral activity in the embryonated eggs. Results: 60 mg/100 ml concentration of Glycyrrhiza extract did not produce any toxicity in the embryonated eggs and showed anti-viral activity against the virus. Similarly, 20 μg/ml ribavirin was non-toxic in the embryonated eggs and contained anti-viral activity. Conclusion: It may conclude from the presented study that 60 mg/100 ml Glycyrrhiza extract inhibits replication of Newcastle disease virus and is non-toxic in the embryonated eggs. So, Glycyrrhiza glabra extract may be further evaluated in future to determine the potentially active compounds for their anti-viral activity against Newcastle disease virus. Furthermore, the mechanism of action of these active phytochemicals as an antiviral agent would be helpful to elucidate the pathogenesis of the disease.

10aAnti-viral agents10aEmbryonated eggs10aGlycyrrhiza glabra10aNewcastle disease virus10aRibavirin1 aOmer, Muhammad, Ovais1 aAlMalki, Waleed, Hassan1 aShahid, Imran1 aKhuram, Shahzada1 aAltaf, Imran1 aImran, Saeed uhttps://www.phcogres.com/article/2014/6/1/1041030974-8490122911