02459nas a2200229 4500008004100000245007900041210006900120260001800189300001100207490000600218520177900224653001702003653002102020653001702041653002102058653001702079100001402096700001802110700001802128700001602146856006702162 2012 eng d00aMoringa Oleifera Lam.: Protease Activity Against Blood Coagulation Cascade0 aMoringa Oleifera Lam Protease Activity Against Blood Coagulation cdecember,2011 a 44-490 v43 a

Background: The present study evaluated the protease activity of aqueous extracts of Moringa oleifera (Moringaceae) leaf (MOL) and root (MOR). Materials and Methods: Protease activity was assayed using casein, human plasma clot and human fibrinogen as substrates. Results: Caseinolytic activity of MOL was significantly higher (P ≤ 0.05) than that of MOR. Similar observations were found in case of human plasma clot hydrolyzing activity, wherein MOL caused significantly higher (P ≤ 0.05) plasma clot hydrolysis than MOR. Zymographic techniques were used to detect proteolytic enzymes following electrophoretic separation in gels. Further, both the extracts exhibited significant procoagulant activity as reflected by a significant decrease (P ≤ 0.05) in recalcification time, accompanied by fibrinogenolytic and fibrinolytic activities; clotting time was decreased from 180 ± 10 sec to 119 ± 8 sec and 143 ± 10 sec by MOL and MOR, respectively, at a concentration of 2.5 mg/mL. Fibrinogenolytic (human fibrinogen) and fibrinolytic activity (human plasma clot) was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), plate method and colorimetric method. Zymographic profile indicated that both the extracts exerted their procoagulant activity by selectively hydrolyzing Aa and Bb subunits of fibrinogen to form fibrin clot, thereby exhibiting fibrinogenolytic activity. However, prolonged incubation resulted in degradation of the formed fibrin clot, suggesting fibrinolytic like activity. Conclusions: These findings support the traditional usage of M. oleifera extracts for wound healing.

10aCaseinolytic10aFibrinogenolytic10aFibrinolytic10aMoringa oleifera10aProcoagulant1 aSatish, A1 aSairam, Sudha1 aAhmed, Faiyaz1 aUrooj, Asna uhttps://www.phcogres.com/article/2012/4/1/1041030974-849091034