01950nas a2200217 4500008004100000245012100041210006900162260001800231300001300249490000600262520122100268653002501489653002801514653002601542653001501568100002301583700002301606700001801629700001701647856006801664 2012 eng d00aProtective Effects of Natsumikan (Citrus natsudaidai) Extract on Acetaminophen-induced Lethal Hepatotoxicity in Mice0 aProtective Effects of Natsumikan Citrus natsudaidai Extract on A cOctober ,2012 a 234-2360 v43 a

Background: Like other citrus fruits, natsumikan (Citrus natsudaidai ) contains several antioxidative nutrients which occur in higher concentrations in the peel than in the pulp. A high dose of acetaminophen (APAP) generates highly reactive intermediates and causes fatal liver injury. In this study, we examined whether an extract from immature natsumikan peel prevents lethal hepatotoxicity induced by a lethal dose of APAP in mice. Materials and Methods: Male ICR mice were treated orally with natsumikan extract (300 and 1,000 mg/kg) 2, 26, and 50 hr before single oral APAP (300 mg/kg) administration. Mice were fasted for 18 hr before APAP treatment, but given tap water ad libitum. Survival was assessed for 24 hr after APAP treatment. Results: Following administration of 300 mg/kg APAP, all mice died within 6 h. However, pretreatment with natsumikan extract (300 and 1,000 mg/kg) or silymarin (300 and 1,000 mg/kg) increased the survival rate to 16.7%, 33.3%, 16.7%, and 50%, respectively, at 24 hr. Conclusion: The results suggest that natsumikan has a protective effect on APAP-induced lethal hepatotoxicity.

10aacetaminophen (APAP)10aHepatoprotective effect10alethal hepatotoxicity10aNatsumikan1 aYamaura, Katsunori1 aNakayama, Noriyuki1 aShimada, Maki1 aUeno, Koichi uhttps://www.phcogres.com/article/2012/4/4/1041030974-8490102274