02728nas a2200241 4500008004100000245011700041210006900158260001600227300001000243490000600253520191400259653003102173653002202204653001302226653001202239100002502251700003102276700003102307700002502338700003302363700002202396856006802418 2013 eng d00aInsulin Secretion Enhancing Activity of Roselle Calyx Extract in Normal and Streptozotocin-induced Diabetic Rats0 aInsulin Secretion Enhancing Activity of Roselle Calyx Extract in cApril 2013 a65-700 v53 a

Background and Objective: Our recent study revealed the antihyperglycemic activity of an ethanolic extract of roselle calyxes (Hibiscus sabdariffa) in diabetic rats. The present study had, therefore, an objective to investigate the mechanism underlying this activity. Materials and Methods: Male Sprague Dawley rats were induced to be diabetes by intraperitoneal injection of 45 mg/kg streptozotocin (STZ). Normal rats as well as diabetic rats were administered with the ethanolic extract of H. sabdariffa calyxes (HS-EE) at 0.1 and 1.0 g/kg/day, respectively, for 6 weeks. Then, blood glucose and insulin levels, at basal and glucose-stimulated secretions, were measured. The pancreas was dissected to examine histologically. Results: HS-EE 1.0 g/kg/day significantly decreased the blood glucose level by 38 ± 12% in diabetic rats but not in normal rats. In normal rats, treatment with 1.0 g/kg HS-EE increased the basal insulin level significantly as compared with control normal rats (1.28 ± 0.25 and 0.55 ± 0.05 ng/ml, respectively). Interestingly, diabetic rats treated with 1.0 g/kg HS-EE also showed a significant increase in basal insulin level as compared with the control diabetic rats (0.30 ± 0.05 and 0.15 ± 0.01 ng/ml, respectively). Concerning microscopic histological examination, HS-EE 1.0 g/kg significantly increased the number of islets of Langerhans in both normal rats (1.2 ± 0.1 and 2.0 ± 0.1 islet number/10 low-power fields (LPF) for control and HS-EE treated group, respectively) and diabetic rats (1.0 ± 0.3 and 3.9 ± 0.6 islet number/10 LPF for control and HS-EE treated group, respectively). Conclusion: The antidiabetic activity of HS-EE may be partially mediated via the stimulating effect on insulin secretion.

10aAntihyperglycemic Activity10aInsulin Secretion10aPancreas10aRoselle1 aWisetmuen, Eamruthai1 aPannangpetch, Patchareewan1 aPannangpetch, Patchareewan1 aKukongviriyapan, Upa1 aYutanawiboonchai, Wiboonchai1 aItharat, Arunporn uhttps://www.phcogres.com/article/2013/5/2/1041030974-8490110520