02936nas a2200253 4500008004100000245005700041210005700098260001800155300001000173490000600183520220400189653001602393653002702409653001702436653003002453653001602483100002002499700002002519700001602539700001502555700002102570700002302591856006802614 2013 eng d00aVolatile Constituents of Pinus Roxburghii from Nepal0 aVolatile Constituents of Pinus Roxburghii from Nepal cJanuary 2013 a43-480 v53 a

Background: Pinus roxburghii Sarg. Is one of 3 species of pine found in Nepal, the oil of which is traditionally used to treat cuts, wounds, boils, and blisters. Objective: To obtain, analyze, and examine the anti-microbial and cytotoxic activities of the essential oils of P. roxburghiiMaterials and Methods: Three plant parts (cone, needle, and bark) of Pinus roxburghii were collected in Biratnagar, Nepal. The essential oils were obtained by hydrodistillation, and the chemical compositions were determined by GC-MS. The needle and cone essential oils were screened for anti-microbial activity against Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Aspergillus niger; brine shrimp (Artemia salina) lethality; and in-vitro cytotoxicity against MCF-7 cells. Results: GC-MS analysis for the cone oil revealed 81 compounds with 78 components being identified (95.5% of the oil) while 98.3% of needle oil was identified to contain 68 components and 98.6% of the bark oil (38 components) was identified. The 3 essential oils were dominated by sesquiterpenes, particularly (E)-caryophyllene (26.8%-34.5%) and α-humulene (5.0%-7.3%) as well as monoterpene alcohols terpinen-4-ol (4.1%-30.1%) and α-terpineol(2.8%-5.0%). The monoterpene δ-3-carene was present only in needle and cone essential oils (2.3% and 6.8%, respectively). Bio-activity assays of the cone essential oil of P. roxburghii showed remarkable cytotoxic activity (100% killing of MCF-7 cells at 100 μg/mL) along with notable brine shrimp lethality (LC50 =11.8 μg/mL). The cone essential oil did not show anti-bacterial activity, but it did exhibit anti-fungal activity against Aspergillus niger (MIC=39 μg/mL). Conclusion: The bioactivity of P. roxburghii essential oil is consistent with its traditional medicinal use.

10aAnti-fungal10aBrine shrimp lethality10acytotoxicity10aEssential oil composition10aα-humulene1 aSatyal, Prabodh1 aPaudel, Prajwal1 aRaut, Josna1 aDeo, Akash1 aDosoky, Noura, S1 aSetzer, William, N uhttps://www.phcogres.com/article/2013/5/1/1041030974-8490105650