02611nas a2200205 4500008004100000245011100041210006900152260001400221300001200235490000600247520191100253653002702164653003102191653002602222653002502248100002202273700002202295700002102317856006702338 2012 eng d00aActive Spermatogenesis Induced by a Reiterated Administration of Globularia Alypum L. Aqueous Leaf Extract0 aActive Spermatogenesis Induced by a Reiterated Administration of cJuly,2012 a138-1470 v43 a

Background: Globularia alypum L. (Globulariaceae) is a shrub growing in the Mediterranean basin and known to be used as a popular medicine for its several pharmacological properties against rheumatism, gout, typhoid, intermittent fever, and diabetes. Materials and Methods: The acute and chronic toxicities of a G. alypum L. aqueous leaf extract were studied in animals. Acute toxicity was performed in male and female mice whereas chronic toxicity was realized in male and female rats that orally received the drug at the doses of 300 and 600 mg/kg/24 h for 30 days. Results: Acute toxicity showed that the extract, administered by the oral route, does not induce any mortality even for a dose of 10,000 mg/kg. Administered by the intra-peritoneal route to female and male mice, the LD50 of the extract was found to be of 2750 and 2550 mg/kg, respectively. A chronic toxicity study showed that, compared to the control groups that only received the vehicle (water), the drugs affects weight growth (effects more pronounced in female than in male rats), some organs weight after autopsy, hematological and biochemical parameters and histology of some principal organs (lungs: histological grades I to II pulmonary hypertension (PHT), respiratory distress syndrome (ARDS), and lymphoid hyperplasia; esophagus: thinning down of esophageal wall, atrophic muscular coat). The most important finding of the study was the recorded active spermatogenesis induced by the reiterated administrations of the drug that was confirmed by reducing the administered dose and the period of treatment (100 mg/kg/24 hr for 15 days). Conclusion: It is suggested that the G. alypum L. leaf extract contains active substances with androgenic properties that could be used in human therapy.

10aActive spermatogenesis10aAcute and chronic toxicity10aAndrogenic properties10aGlobularia alypum L.1 aFehri, Badreddine1 aAiache, Jean-Marc1 aKK, Mueen, Ahmed uhttps://www.phcogres.com/article/2012/4/3/1041030974-849099073