02718nas a2200217 4500008004100000245009800041210006900139260001800208300001000226490000600236520200100242653004002243653003002283653002302313653001302336100002002349700001802369700001702387700002802404856006802432 2013 eng d00aProtective Effects of Picrorhiza Kurroa on Cyclophosphamide-induced Immunosuppression in Mice0 aProtective Effects of Picrorhiza Kurroa on Cyclophosphamideinduc cJanuary 2013 a30-350 v53 a

Objective: To study the immunomodulatory effect of ethanolic and aqueous extract of the rhizomes of Picrorrhiza kurroa (Scrophulariaceae) in normal and immunosuppressed mice models. Materials and Methods: The rhizomes extract of Picrorrhiza kurroa was administered orally according to their body weight in mice. The study was carried out by various hematological and serological tests. The assessment of immunomodulatory activity on specific and non-specific immunity was studied by administration of test extract. The method of cyclophasphamide-induced immunosuppression was employed with slight modification to study the immunomodulatory potential of the extract. Plant extracts were administered by oral feeding canula to the test groups (groups III-VI), group I (control animals) and group II (model control animals) received same volume of normal saline (0.2 ml). Humoral antibody response to SRBC measurement of antibody titer by hemagglutination reaction was done. The mice belonging to the all groups were antigenically challenged with SRBC (0.5x10 9 cells/ml/100 g) on 10 th day intraparitoneally. Cellular immune response (Foot pad reaction test) the edema was induced by injecting SRBC (0.025x10 9 cells) in left paw, and 0.025 ml of saline was injected in right paw. Results: The plant extract showed protective effects on humoral immunity. The change in percentage deduction in footpad volume was also found significant (P<0.001). Administration of extract remarkably ameliorated both cellular and humoral antibody response. Conclusion: It is concluded that the test extracts possessed promising immunostimulant properties. But, the alcoholic extract is more potent than aqueous extract in producing delayed type hypersensitivity response.

10aHematological and Serological Tests10aImmunomodulatory Activity10aPicrorrhiza Kurroa10aRhizomes1 aHussain, Arshad1 aShadma, Wahab1 aMaksood, Ali1 aAnsari, Shahid, Hussain uhttps://www.phcogres.com/article/2013/5/1/1041030974-8490105646