02299nas a2200241 4500008004100000245015400041210006900195260001400264300001200278490000600290520145200296653002401748653001901772653002001791653002301811100003101834700002101865700002101886700002801907700002701935700002801962856006701990 2012 eng d00aAcute toxicity impacts of Euphorbia hirta L extract on behavior, organs body weight index and histopathology of organs of the mice and Artemia salina0 aAcute toxicity impacts of Euphorbia hirta L extract on behavior  cJuly,2012 a170-1770 v43 a

Background: The methanol extract of Euphorbia hirta L (Euphorbiaceae), which is used in traditional medicines, was tested for in vivo toxicity. Materials and Methods: In vivo brine shrimp lethality assay and oral acute toxicity study at single high dose of 5000 mg/kg and observation for 14 days in mice were used to study the toxic effect of E. hirta. Results: Brine shrimp lethality assay was used to calculate the median lethal concentration (LC 50 ) of E. hirta (for leaves, stems, flowers and roots) methanolic extracts at concentrations from 100 to 0.07 mg/ml. The LC 50 values of 1.589, 1.420, 0.206 and 0.0827 mg/ml were obtained for stems, leaves, flowers and roots, respectively. Potassium dichromate (the positive control) had LC 50 value of 0.00758 mg/ml. The acute oral toxicity study of the leaf extract resulted in one third mortality and mild behavioral changes among the treated mice. No significant statistical differences found between body weight, relative (%) and absolute (g) organ weights of treated and untreated groups (P> 0.05). Gross and microscopic examination of the vital organ tissues revealed no differences between control and treated mice. All the tissues appeared normal. Conclusion : E. hirta leaves methanol extract has exhibited mild toxic effects in mice.

10aAcute oral toxicity10aArtemia salina10aEuphorbia hirta10aMethanolic extract1 aRajeh, Mohammad, Abu Basma1 aKwan, Yuet, Ping1 aZakaria, Zuraini1 aLatha, Lachimanan, Yoga1 aJothy, Subramanion, L.1 aSasidharan, Sreenivasan uhttps://www.phcogres.com/article/2012/4/3/1041030974-849099085