02415nas a2200229 4500008004100000245011100041210006900152260001700221300001200238490000600250520167100256653001401927653002701941653002201968653002401990100001502014700001902029700002802048700001602076700002502092856006802117 2015 eng d00aPreclinical screening of phyllanthus amarus ethanolic extract for its analgesic and antimicrobial activity0 aPreclinical screening of phyllanthus amarus ethanolic extract fo cOctober 2015 a378-3840 v73 a

Background: To discover a new agent which possesses dual property of analgesic and antimicrobial activity, thereby reducing the burden of polypharmacy. Phyllanthus amarus was screened for its analgesic and antimicrobial activities. Objectives: The objective was to evaluate the analgesic and antimicrobial activity, of P. amarus ethanolic extract (PAEE). Materials and Methods: The ethanolic extract of P. amarus was prepared using Soxhlet apparatus. An in vivo study using Swiss albino mice was done to screen the central and peripheral analgesic activity of P. amarus extract. The extract was administered at a dose of 100 mg/kg body weight orally. The peripheral analgesic activity was assessed using acetic acid induced writhing test. The central analgesic activity was assessed using Eddy’s hot plate apparatus. An in vitro study was carried out to study the antimicrobial activity of the above extract using selected species of Streptococcus mutans, and S. salivarius. The antimicrobial activities were determined using the agar well method. Results: The ethanolic extract of P. amarus showed significant (P < 0.05) peripheral and central analgesic activity. In vitro antimicrobial screening indicated that the ethanolic extract had shown a zone of inhibition against S. mutans and S. salivarius in the agar wells. Conclusion: This study showed that PAEE exhibited significant analgesic and antimicrobial activities.

10aAnalgesic10aAntimicrobial activity10aEthanolic extract10aPhyllanthus amarus.1 aBhat, Sham1 aHegde, Sundeep1 aChandrashekhar, Sharath1 aRao, S., N.1 aManikkoth, Shyamjith uhttps://www.phcogres.com/article/2015/7/4/1041030974-8490159577