02500nas a2200241 4500008004100000245016500041210007300206260001800279300001200297490000600309520166800315653001901983653001602002653001402018653005102032653002002083653001002103100002502113700001902138700001702157700001902174856006502193 2017 eng d00aDevelopment and Validation of High‑performance Thin‑layer Chromatography Method for Simultaneous Determination of Polyphenolic Compounds in Medicinal Plants0 aDevelopment and Validation of High‑performance Thin‑layer Chroma cDecember 2017 as67-s730 v93 a

Context: Quantitative standardization of plant-based products is challenging albeit essential to maintain their quality. Aims: This study aims to develop and validate high-performance thin-layer chromatography (HPTLC) method for the simultaneous determination of rutin (Ru), quercetin (Qu), and gallic acid (Ga) from Psidium guajava Linn. (PG) and Aegle marmelos (L.) Correa. (AM) and correlate with antioxidant activity. Materials and Methods: The stock solution (1 mg/mL) of standard Ru, Qu, and Ga in methanol: Water (1:1) was serially diluted and spotted (5 μL) on slica gel 60 F254thin-layer chromatography plates. Toluene: Ethyl acetate: Formic acid: Methanol (3:4:0.8:0.7, v/v/v) was selected as mobile phase for analysis at 254 nm. Hydroalcoholic (1:1) extracts of leaves of PG and AM were fractionated and similarly analyzed. Antioxidant activity was also determined using 2, 2-diphenyl-1-picrylhydrazyl assay. Results: The developed method was robust and resolved Ru, Qu, and Ga at Rf0.08 ± 0.02, 0.76 ± 0.01, and 0.63 ± 0.02, respectively. The intra-day, interday precision, and interanalyst were <2% relative standard deviation. The limit of detection and limit of quantification for Ru, Qu, and Ga were 4.51, 4.2, 5.27, and 13.67, 12.73, 15.98 ng/spot, respectively. Antioxidant activity (Log 50% inhibition) of PG and AM was 4.947 ± 0.322 and 6.498 ± 0.295, respectively. Conclusion: The developed HPTLC method was rapid, accurate, precise, reproducible, and specific for the simultaneous estimation of Ru, Qu, and Ga.

10aAegle marmelos10aGallic acid10aGuercetin10aHigh‑performance thin‑layer chromatography10aPsidium guajava10aRutin1 aNair, CV, Jayachandr1 aAhamad, Sayeed1 aKhan, Washim1 aAnjum, Varisha uhttps://www.phcogres.com/article/2017/9/5s/104103prpr12216-0