02371nas a2200277 4500008004100000245011800041210006900159260001700228300001200245490000600257520152400263653000601787653002801793653001901821653001701840653001201857653001201869653001901881100002901900700001901929700002301948700001601971700002101987700001702008856006802025 2015 eng d00aAntiproliferative evaluation of terpenoids and terpenoid coumarins from Ferulago macrocarpa (Fenzl) Boiss. fruits0 aAntiproliferative evaluation of terpenoids and terpenoid coumari cOctober 2015 a322-3280 v73 a

Background: Ferulago macrocarpa is a plant used as flavoring agent and protectant in the food industry and as a folk medicinal plant in Iran with no available information on its chemical identity. Ferulago spp. showed to contain biologically terpenoids and coumarins. Objectives: The objective was to isolate and characterize terpenoids and coumarins from the acetone extract of F. macrocarpa fruits and to evaluate their antiproliferative effects on several cell lines. Materials and Methods: A series of normal and reverse phase gravity and high‑performance liquid chromatography analyses were used to purify constituents. Compounds 1–5 and 7 were evaluated for their cytotoxic effects on MCF‑7, HT‑29 and H‑1299 cell lines. Results: Six compounds including bornyl acetate (1), 1,10‑di‑epi‑cubenol (2), stigmasterol (3) and three coumarins grandivittin (4), prantschimgin (5) and 4”‑hydroxygrandivittin (7) along with mixtures of feruloyl derivatives (6a‑6c) have been purified. Their structures were established by spectroscopic methods including nuclear magnetic resonance and MS analyses. Compound 2 showed moderate cytotoxicity effect with IC50 values of 5.0 and 6.7 μM on MCF‑7 and HT‑29, respectively. Conclusion: 1,10‑di‑epi‑Cubenol could be considered as a potential proliferation inhibitor of MCF‑7 and HT‑29 cell lines.

10a110a10‑di‑epi‑Cubenol10aBornyl acetate10aGrandivittin10aHT‑2910aMCF‑710aPrantschimgin.1 aSajjadi, Seyed‑Ebrahim1 aJamali, Maryam1 aShokoohinia, Yalda1 aAbdi, Gisya1 aShahbazi, Behzad1 aFattahi, Ali uhttps://www.phcogres.com/article/2015/7/4/1041030974-8490158437